Identification of Water Soluble Metabolites of Pentachlorophenol(PCP) in the Suspension Cultures of Soybean and Rice Cells;2. Isolation and characterization of PCP glucose conjugates

콩과 벼 현탁배양시(懸濁培養時) PCP 수용성대사물(水溶性代謝物)의 동정(同定);2. PCP glucose conjugates의 분리(分離) 및 분석(分析)

  • Kim, Pil-Je (Department of Agricultural Chemistry, College of Agricultural & Life Science, Seoul National University) ;
  • Park, Chang-Kyu (Department of Agricultural Chemistry, College of Agricultural & Life Science, Seoul National University)
  • 김필제 (서울대학교 농업생명과학대 농화학과) ;
  • 박창규 (서울대학교 농업생명과학대 농화학과)
  • Published : 1996.03.31

Abstract

Abstracts From the previous metabolic study of Pentachlorophenol(PCP), PCP was found to be exclusively transformed into ${\beta}-glucose$ conjugates of PCP in soybean and rice cell suspension cultures. In order to gather structural information of of the glucose conjugate, their aglycons and glycon have been analyzed by GC and GC/MS respectively, after thorough purification by chromatographic techniques. The glucose conjugates were effectively purified through a 1-butanol extraction followed by Silica gel TLC, Sephadex column chromatography and HPLC. Aglycons of the metabolites were identified as PCP, isomeric mixture of tetrachlorophenol, and tetrachlorocatechol and glycon were identified as glucose, suggesting that there are at least three kinds of glucose conjugates with different phenolic moieties. Under controlled conditions, the glucose conjugates were separated into three HPLC peaks which released respective aglycon upon a hydrolytic treatment. These results give valuable information on the structure of the glucose conjugates such that some PCP-driven chlorophenols, in addition to PCP, are also conjugated with glucose.

Keywords

PCP;glucose conjugate;tetrachlorocatechol;chlorophenols