Fixation of Cellular Ultrastructure by the Microwave Irradiation

마이크로파 조사에 의한 세포 미세구조의 고정 효과

  • 신길상 (순천향대학교 자연과학대학 생명과학부) ;
  • 김완종 (순천향대학교 자연과학대학 생명과학부) ;
  • 전진석 (계명대학교 자연과학대학 생물학과)
  • Published : 1996.12.01

Abstract

The microwave fixator has recently been introduced in morphological research. The present study was carried out to investigate the ultrastructural effects of microwave fixation of rat brain. kidney, liver and skeletal muscle tissues. The results are as follows: In the case of microwave fixed cerebrum. the cytoplasmic processes of neurons and the various membranous organelles such as nuclear envelope, mitochondria, rough endoplasmic reticulum and Golgi apparatus were well preserved, The myelin sheath wrapping neuronal axon was prominent. Microwave fixed hepatocytes showed the microvilli on the free surface of bile canaliculus, the evident nucleolar components, and typical organelles. In nephron, ultrastructures of glomerulus and Bowman's capsule were preserved, and also tubular wall were structurally observed. Among the skeletal muscle cells, plentiful collagen fibers were appeared, myofibrils and mitochondria were typically observed. In conclusion, the microwave fixation procedures result in an good preservation of the tissues and would be time- and reagent-saving.