- Volume 26 Issue 4
Fixation of Cellular Ultrastructure by the Microwave Irradiation
마이크로파 조사에 의한 세포 미세구조의 고정 효과
- Shin, Kil-Sang (Department of Biology, Soonchunhyang University) ;
- Kim, Wan-Jong (Department of Biology, Soonchunhyang University) ;
- Jeon, Jin-Seok (Department of Biology, Keimyung University)
- Published : 1996.12.01
The microwave fixator has recently been introduced in morphological research. The present study was carried out to investigate the ultrastructural effects of microwave fixation of rat brain. kidney, liver and skeletal muscle tissues. The results are as follows: In the case of microwave fixed cerebrum. the cytoplasmic processes of neurons and the various membranous organelles such as nuclear envelope, mitochondria, rough endoplasmic reticulum and Golgi apparatus were well preserved, The myelin sheath wrapping neuronal axon was prominent. Microwave fixed hepatocytes showed the microvilli on the free surface of bile canaliculus, the evident nucleolar components, and typical organelles. In nephron, ultrastructures of glomerulus and Bowman's capsule were preserved, and also tubular wall were structurally observed. Among the skeletal muscle cells, plentiful collagen fibers were appeared, myofibrils and mitochondria were typically observed. In conclusion, the microwave fixation procedures result in an good preservation of the tissues and would be time- and reagent-saving.