Effect of the Toluene Administration on the Liver Damage in Rats Fed Low or Standard Protein Diet

저 및 표준단백식이로 성장시킨 흰쥐에 Toluene 투여가 간 손상에 미치는 영향

  • 윤종국 (계명대학교 공중보건학과) ;
  • 김경순 (계명대학교 공중보건학과) ;
  • 임영숙 (대구공업전문대학 식품영양과)
  • Published : 1996.12.01


To study the effect of toluene administration on the liver damage, rats were previously fed a low (casein 7%, LP) or standard(casein 20%, SP) protein diet and for four days toluene(50% in olive oil) was given at 0.2ml/100g body weight/day to the male rats, and then the degree of liver damage in toluenetreated animals fed LP were compared with those fed SP. The increasing rate of liver weight/body weight and the serum levels of xanthine oxidase to the control group were higher in rats fed SP than those fed LP. The decreasing rate of protein contents in cytosol, mitochondria and glycogen, glutathione contents of liver to the control group were higher in rats fed SP than those fed LP. In histopathological findings, the swelling of hepatic cell around the central vein was demonstrated in all the two groups toluene-treated rats. But the degree of swelling severity in hepatocytes was somewhat higher in rats fed SP than those fed LP. Therefore it is assumed that the degree of liver damage severity in toluenetreated animals was higher in rats fed SP than those fed LP.

식이성 단백질 함량이 toluene에 의한 간 손상에 어떠한 영 향을 미치는지를 검토할 목적으로 흰쥐를 저 단백식이 (casein 7%, LP) 및 고단백식이 (casein 20%, SP)로 성장시킨 후 체중 100g당 toluene과 olive oil의 동량흔액 0.2ml를 1일 1회 4번 투여한 다음 24시간 후 처치하였다. 체중 100g당 간무게는 SP군이 대조군에 비하여 약 28%, LP군은 약 17%의 유의한 증가를 보였으며 혈청 xanthine oxidase(XO)활성은 SP군에서는 약 35%의 유의한 증가를 나타내었으나 LP군에서도 증가되었으나 유의성은 없었다. 한편 toluene투여로 인한 간 glycogen, glutathione(GSH) 함량 감소율에 있어서는 SP군이 LP군 보다 높게 나타났으며 간세포의 대조군에 대한 cytosol, mitochondria 단백함량 감소율 역시 SP군이 LP군 보다 증가되는 경향을 보였다. 그리고 병리조직검사 소견에서는 SP군에서 swelling 정도가 LP군에 비하여 심화되어 나타남을 알 수 있었다.



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