Synergistic Effect in Mosquitocidal Activity of Crystal Proteins from Bacillus thuringiensis NT0423 Transformed with cryIVD Gene

cryIVD 유전자로 형질전환된 Bacillus thuringiensis NT0423 균주 내독소 단백질의 모기 유충에 대한 독성의 상승효과

  • 박현우 (서울대학교 농생물학과 및 농업생물신소재연구센터) ;
  • 유용만 (서울대학교 농생물학과 및 농업생물신소재연구센터) ;
  • 오현우 (KIST 생명공학연구소) ;
  • 진병래 (서울대학교 농생물학과 및 농업생물신소재연구센터) ;
  • 김인기 ((주)선양) ;
  • 강석권 (서울대학교 농생물학과 및 농업생물신소재연구센터, Corresponding)
  • Published : 1996.03.01

Abstract

Bacillus thuringiensis NT0423 produces quite a typical bipyramidal crystals of a common major band of ca. 130 kDa, and has dual specificity against Lepidoptera and Diptera. To enforce the Diptera-toxicity of B. thuringiensis NT0423, cryND gene was transformed 30 B. thuringiensis NT0423. The transfonnant B. thuringiensis PT1227 was obtained from introduction of pCGl0 into B. thuringiensis NT0423 by electroporation. The result showed that cryND and resident crystal protein genes in transformant were stably expressed with its own shape. Furthermore, the toxicity of B. thuringiensis PT1227 against Diptera was highly enforced, suggesting that the enforced toxicity of B. thuringiensis PT1227 was due to synergistic effect of both introduced and resident crystal proteins in transformant.