Role of general esterases in deltamethrin resistance mechanism of diamondback moth, Plutrlla xylostella L.

배추좀나방(Pulltella xylostella L.)의 deltamethrin 저항성 기작에 관한 에스테라제의 역할

  • 김용균 (안동대학교 자연과학대학 농생물학과) ;
  • 장동걸 (안동대학교 자연과학대학 농생물학과)
  • Published : 1996.03.01


General esterases were analysed quantitatively and qualitatively to see their role in deltamethrin resistance mechanisms of the diamondback moth, Plutella xylostella L. Selection with 0.1 g of deltamethrin in each generation induced the moth to decrease susceptibility to the insecticide and to increase esterase activities of the fourth instar larvae. Both characters were highly correlated so that the correlation coefficient (r) between LDSo @g) of deltamethrin and esterase activities (~M/min/pg) was 0.9918 (P=0.0082). Nondenaturing PAGE (6%) separated 17 esterase bands from the whole body extracts of the fourth instar larvae. Deltamethrin-selected populations had fewer esterase bands than had the unselected. Four esterase bands (E3, E4, Ell, and E13) were, however, specific to deltamethrin-selected populations.