Characterization of Synechocystic PCC6803 transformed with cryIVD gene of Bacillus thuringiensis subsp. morrisoni PG-14 and its mosquitochidal effect on Anopheles sinensis

Bacillus thuringiensis subsp. morrisoni PG-14 cryIVD 유전자로 형질전환된 Synechocytis PCC6803의 특성과 학질모기에 대한 살충효과

  • 이대원 (서울대학교 농생물학과) ;
  • 박현우 (서울대학교 농생물학과) ;
  • 김호산 (서울대학교 농생물학과) ;
  • 진병래 (서울대학교 농생물학과) ;
  • 유효석 (서울대학교 농생물학과) ;
  • 김근영 (농촌진흥청 잠사곤충연구소) ;
  • 강석권 (서울대학교 농생물학과)
  • Published : 1996.03.01

Abstract

For the effective control of mosquito larvae, Anopheles sinensis, the expression vector pCYASK5-1 containing cryIVD gene of Bacillus thuringiensis subsp. morrisoni PG-14 was constructed and transformed into the cyanobacterium Synechocystis PCC6803. The transformants were selected on BG-11 medium containing kanamycin. The expression of cryIVD gene in transformant was confirmed by SDS-PAGE and Western blot analysis. The mortality of A. sinensis larvae was scored for 3 days. Furthermore, growth and distribution rate of transformant were examined. The results showed that Synechocystis PCC6803 transformed with cryIVD gene of B. thuringiensis subsp. morrisoni PG-14 was highly toxic to A. sinensis larvae, demonstrating that it will be a potential agent for mosquito control.