Effect of Dietary Protein Level on Immune Substances in Milk and its Transfer to Pups in Rats

흰쥐에서 식이 단백질 수준이 수유를 통한 수동면역에 미치는 영향

  • 김화영 (이화여자대학교 가정과학대학 식품영양학과)
  • Published : 1996.07.01

Abstract

This study was performed to determine the effect of maternal protein intake on 1) the concentration of immune substances in milk 2) degree of passive immunity to pups via lactation, and 3) specific antibody production to a specific antigen, $\beta$-lactoglobulin(BLG). 4) the effect of passive immunity that pups received from mother during lactation on the production of antibodies when the pups were challenged to the same antigen. Part of the female rats were immunized with BLG before and during pregnancy. The pregnant rats were placed into either 25% or 10% isolated soy protein diet throughout gestation and lactation. After weaning, pups from each group continued to be fed the same diet. At 18 weeks of age, all the pups were challenged with BLG. Total IgA and IgG, lysozyme, BLG-specific IgA and IgG were measured in dam's serum, dam's milk, and pup's serum. Total IgG, and lysozyme in dam's serum and milk were higher in high protein group. Total IgA and IgG in pup's serum remained higher in high protein group from 5 to 18 weeks of age. BLG-specific antibodies were found in the milk and serum of immunized dams, and in serum of pups born to immunized dams but not in the non-immunized group. BLG-specific IgA and IgG were again higher in high protein group and declined with time. The concentration decreased faster in the low proetein group than in the high protein groups. After immunization the pups with LBG, serum BLG-specific antibodies were not differ between rats born to immunized dams and those born to non-immunized dams. Therefore passive immunity rats received via milk as a pup had no effect on the BLG-specific antibody production later in life. This study shows the importance of protein status of mother and strongly support to the endorsement of breast feeding.

References

  1. 한국영양학회지 v.25 영양상태와 면역능력 김화영
  2. Am J Clin Nutr v.53 Nutrition and immunity : lessons from the past and new insights into the future Chandra,R.K.
  3. J Nutr v.122 Protein-energy malnutrition and immunological responses Chandra,R.K.
  4. Am J Clin Nutr v.37 Effect of maternal nutritional status on immunological substanes in human colostrum and milk. Miranda,R.;Saravia,N.G.;Ackerman,R.;Murphy,N.;McMurray,D.N.
  5. Am J Clin Nutr v.51 Antimicrobial proteins of maternal and cord sers and human milk in relationto maternal nutritional status Chang,S.J.
  6. 한국영양학회지 v.27 임산부의 영양상태와 모유의 면역물질함량에 관한 연구 김화영;김영나;김순미
  7. Proc Soc Exp Biol Med v.148 Rat immunoglobulin in serum and secretions : Comparision of IgM, IgA and IgG in serum, colostrum, milk and saliva of protein malnourished and normal rats Michalek,S.M.;Rahman,A.F.R.;Mcghee,J.R.
  8. Ann N Y Acad Sci v.409 Colostrum derived immunity and maternal-neonatal interaction Orga,P.L.;Losonsky,G.A.;Fishaut,M.
  9. Ann Allergy v.53 The immune respenses of the mammary gland and its significance for the neonate Hanson,L.;Ahlstedt,S.;Andersson,B.;Cruz,J.R.
  10. J Immunol v.141 Effects of early antigen exposure through lactation on later specific antibody responses in mice Komatsu,T.;Okao,M.;Miyamoto,H.;Chen,T.;Shinka,S.
  11. Nutr Rev. v.45 Banked milk is low in immunologically protective proteins
  12. Acta Paediatr Scand v.66 Antimicrobial factors in human milk Reddy,V.;Bhaskaram,C.;Raghuramuhi,N.;Jagadeesan,V.
  13. Am J Clin Nutr v.53 Lysozyme, lactoferrin, and secretory immunoglobulin A content in breast milk : Influence of duration of lactation, nutrition status, prolactin status, and parity of mother Hennart,P.F.;Brasseur,D.J.;Desnoeck,J.B.D.
  14. Science v.190 Antibody formation in first and second generation offspring of nutritionally deprived rats. Chandra,R.K.
  15. Int Allergy appl Immun v.78 investigation of the allergenicity of β-lactoglobulin and its cleavage fragments Huang,Q.;Coleman,J.W.;Stanworth,D.R.
  16. Int Archs Allergy appl Immun v.101 Serum antibody response elicited by a casein diet is direcdted to only limited determinants of α s1-casein Kim,S.M.;Enomoto,A.;hachimura,S;Yamauch,K.;Kaminogana,S.
  17. J Nutr v.122 Postnatal selenium repletion protects lungs of neonatal rats from hyperoxia Kim,H.Y.;Picciano,M.F.;Wallig,M.A.
  18. J Biol Chem v.193 Protein Measurment with the Folin Phenol Reagent Lowry,O.H.;Gosebrough,N.J.;Farr,A.L.;Randall,R.J.
  19. 免疫學寶驗入門 松橋直;成內秀雄
  20. J Exp Med v.24 Serum and urinary lysozyme (muramidase) in monocytic and monmyelocytic leukemia Osserman,E.F.;Lawlor,D.P
  21. Acta Padiatr Scand v.80 MemoryT cells in human breast milk Bertotto,A.;Castellucci,G.;Scalise,F.;Tognellini,R.;Vaccaro,R.
  22. Immunology v.79 Immunoglobulin G binding sites on the human foetal intestine : A possible mechanism for the passive transfer of immunity from mother to infant Israel,E.J.;Simister,N.;Freiberg,E.;Caplan,A.;Walker,W.A.
  23. Am J Clin Nutr v.24 Host resistance to infection Mata,L.J.;Wyatt,R.G.