Effects of the Anabolic Steroid, Nandrolone Pheylpropionate, on Growth and Muscle Protein Metabolism in ACTH-treated Rats

ACTH를 투여한 흰쥐에서 아나보릭스테로이드인 Nandrolone Phenylpropionate가 성장한 근육단백질 대사에 미치는 영향

  • 주종재 (군산대학교 식품영양학과)
  • Published : 1996.10.01

Abstract

The effects of an anabolic steroid, nandrolone phenylpropionate(NPP), on body weight gain and body protein, and muscle protein metabolism were inestigated in adrenocorticotrophic hormone(ACTH)-treated male and female rats. Daily injections of 100ug/day of ACTH for 7-8 days caused a cessation of growth in females and a net loss of body weight in males which were associated with significant reductions in body protein content. However, food intake was not affected by ACTH in either sex. The weight, protein content and fractional rate of protein synthesis, measured in vivo, of gastrocnemius muscle were all significantly reduced in both sexes. NPP at a dose of 4mg/kg body weight prevented the reduction in body weight gain in ACTH-treate females but not in males. However, boy protein content was increased by NPP in both sexes which was associated with increases in the weight, protein content and fractional rate of protein synthesis of gastrocnemius muscle. ACTH treatment caused a marked increase in plasma concentrations of corticosterone in both sexes. NPP suppressed much of the increases in corticosterone concentrations in both sexes. The results of the present study suggest that NPP exerts at least part of its anabolic effect by reducing plasma concentrations of catabolic glucocorticoid hormones, through suppressing the response of the adrenals to ACTH.

References

  1. Anim Prod v.2 The effect of diethylstilbestrol, hexoestrol and testosterone on the growth rate and carcass quality of fattening beef steers Burgess,T.D.;Lamming,G.E.
  2. J Anim Prod v.12 Alteration of daily gain, feed efficiency and carcass characteristics in beef cattle with male hormones Burris,M.J.;Bogart,R.;Oliver,A.W.
  3. Pharmacol Ther Bull v.1 Definition of androgens and protein anabolic steroids Kochakian,C.D.
  4. J Anim Sci v.11 The effects of diethylstilbestrol, testosterone and progesterone on growth and fattening and cartain carcass characteristics of western lambs O'Mary,C.C.;Pope,A.L.;King,G.T.;Shelton,J.M.
  5. Ann Neurol v.20 Effect of testosterone on muscle protein synthesis in myotonic dystrophy Griggs,R.C.;Halliday,D.;Kingston,W.;Moxley,R.T.
  6. J Appl Physiol v.66 no.1 Effect of testosterone on muscle mass and muscle protein synthesis Griggs,R.C.;Kingston,W.;Jozefowicz,R.F.;Herr,B.E.;Forbes,G.;Halliday,D.
  7. Science and sporting performance : Management or Manipulation What are the effects of anabolic steroids? Hervey,G.R.;Davies,B.(ed.);Thomas,G.(ed.)
  8. Nutr Abstr Rev v.51 no.8 Effect of hormones on the growth and body composition of animals Galbraith,H.;Topps,J.H.
  9. Livestock Prod Sci v.12 Hormonal systems regulating growth Spencer,G.S.G.
  10. Acta Endocrinol Suppl v.271 Deca-durabolin and parenteral nutrition in post-traumatic patients Mosebach,K.O.;Hausmann,D.;Caspari,R.;Stoeckel,H.
  11. J Trauma v.22 Effect of an anabolic steroid on nitrogen balance and amino acid patterns after total hip replacement Michelsen,C.B.;Askanazi,J.;Kinney,J.M.;Gump,F.E.;Elwyn,D.H.
  12. Br J Clin Pract v.27 The effect of an anabolic steroid on postoperative nitrogen balance Tweedle,D.;Walton,C.;Johnston,I.D.A.
  13. Ann Neurol v.20 Effect of testosterone on muscle protein synthesis in myotonic dystrophy Griggs,R.C.;Halliday,D.;Kingston,W.;Moxley,R.T.
  14. Eur J Biochem v.111 Characterization and quantification of the adnrogen and glucocorticoid receptors in cytosol from rat skeletal muscle Snochowski,M.;Dahlberg,E.;Gustaffson,J.A.
  15. Anim Prod v.26 A note on the effect of castration on the growth performance and concentrations of some blood metabolites and hormones in British Friesian male cattle Galbraith,H.;Dempster,D.G.;Miller,T.B.
  16. Am J Physiol v.229 Interaction of anabolic steroids with glucocorticoid receptor sites in rat muscle cytosol Mayer,M.;Rosen,F.
  17. Kor J Nutr v.29 no.8 Inhibition of corticosterone-induced muscle atrophy and reduction in protein synthesis by the anabolic steroid nandrolone phenylpropionate in femalerats Choo,J.J.
  18. J Endor v.126 Effects of trenbolone acetate and testosterone on growth and on plasma concentrations of corticosterone and ACTH in rats Sillence,M.N.;Rodway,R.G.
  19. Proc Nut Soc v.41 Suppression of adrenocortical function in rats and sheep treated with the anabolic steroid trenbolone acetate Thomas,K.M.;Rodway,R.G.
  20. Biochem J v.178 Effect of glucocorticoid administration on the rate of muscle protein breakdown in vivo in rats, as measured by urinary excretion of 3-methylhistidine Tomas,F.M.;Munro,H.;Young,V.R.
  21. Biochem J v.192 A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of[³H]phenylalanine Garlick,P.J.;McNurlan,M.A.;Preedy,V.R.
  22. Anal Biochem v.134 Improved liquid chromatographic determination of serum cortisol with double internal strandardisation compared to radioimmnoassay and fluorimetry, and evaluation by isotope dilution/mass spectrometry Lamert,W.E.;DeSlypere,J.M.;Jonchheere,J.A.;Vermeulen,A.;DeLeenheer,A.P.
  23. Anal Biochem v.108 Determination of prednisolone, prednisone, and cortisol in human plasma by high-performance liquid chrmatography Scott,N.R.;Chakraborty,J.;Marks,V.
  24. Diabetes/Metab Rev v.4 Endocrine regulation of protein breakdown in skeletal muscle Kettelhut,I.C.;Wing,S.S.;Goldberg,A.J.
  25. Endokrinologie v.79 no.2 Sex differences in adrenocortical structure and function Malendowicz,L.;Mlynarczyk,W.
  26. Anim Prod v.44 Adrenal function in lambs treated with androgenic and oestrogenic growth stimulants Sillence,M.N.;Thomas,K.M.;Anil,H.;Redfern,E.J.;Rodway,R.G.
  27. J Endocr v.98 Effects of trenbolone acetate on adrenal function and hepatic enzyme activities in female rats Thomas,K.M.;Rodway,R.G.
  28. Proc Nut Soc v.44 The relation between sex differences in growth response to trenbolone acetate and the suppression of adrenal activity in male and female rats Sillence,M.N.;Girling,T.R.;Loretto,E.A.;Parry,K.;Taylor,I.G.;Rodway,R.G.
  29. J Cli Endocr Metab v.66 Effect of androgen on adrenal steroidogenesis in normal women Vermesh,M.;Silva,P.D.;Rosen,G.F.;Vijod,A.G.;Lobo,R.A.
  30. Proc Soc Exp Biol Med v.98 Failure of 17α-ethyl-19-nortestosterone to effect plasma 17-hydroxycorticosteroids and ACTH responsiveness in man Cart,A.C.;Weisenfeld,S.;Goldner,M.G.
  31. J Endocr v.25 Effect of an anabolic steroid(methandienone) on pituitary-adrenal function in the human Wynn,V.;Landon,J.;James,V.H.T.
  32. Acta Endocrinologica Supplementum v.271 Deca-durabolin and parenteral nutrition in post-traumatic patients Mosebach,K.O.;Hausmann,D.;Caspari,R.;Stoeckel,H.
  33. Acta Endocrinologica v.89 Effect of glucocorticoids on plasma testosterone in men Schaison,G.;Durand,F.;Mowszowicz,I.
  34. Acta Endocrinologica Supplementum v.214 Biochemical substrate of androgenic actions at a cellular levels in prostate, bulbocavernosus/levator ani and in skeletal muscle Krieg,M.;Voigt,K.D.