Inhibition of Corticosterone-induced Muscle Protein Synthesis by the Anabolic Steroid Nandrolone Phenylpropionate in Female Rats

아나보릭스테로이드인 Nandrolone Phenylpropionate가 암컷 쥐에서 코티코스테론에 의해 야기된 근육단백질 쇠퇴와 근육단백질 합성율 감소에 미치는 영향

  • 주종재 (군산대학교 식품영양학과)
  • Published : 1996.10.01

Abstract

This study was undertaken to determine whether the anabolic steroid nandrolone phenylpropionate(NPP) can inhibit the muscle atrophy and reduction in muscle protein synthesis caused by glucocorticoids in female rates. Daily injections of 50mg/kg of corticosterone for eight days induced significant reductions in body weight gain and protein without affecting food intake. The mass, protein and RNA content, ratio of RNA to protein, and fractional rate of protein synthesis, measured in vivo, of gastrocnemius muscle were all significantly reduced by corticosterone treatement. Simultaneous administration of NPP at a dose of 10mg/kg with corticosteorne (50mg/kg) fully inhibited the reductions in the mass, protein and RNA content of gastrocnemius muscle, and body weight gain and protein with no alteration in food intake but the reduction in fractional rate of muscle protein syntheis was only partially prevented. The results indicate that the anabolic steroid nandrolone phenylpropionate is capable of preventing muscle atrophy in female rats treated with excess corticosterion.

References

  1. Eur J Biochem v.111 Characterization and quantification of the adnrogen and glucocorticoid receptors in cytosol from rat skeletal muscle Snochowski,M.;Dahlberg,E.;Gustaffson,J.A.
  2. Endocrinology v.114 Relative binding affinity of anabolic-androgenic steroids : comparison of the binding to the androgen receptors in skeletal muscle and in prostate, as well as to sex hormone-binding globulin Saartok,T.;Dahlberg,E.;Gustaffson,J.A.
  3. Anim Prod v.26 A not on the effect of castration on the growth performance and concentrations of some blood metabolites and hormones in British Friesian male cattle Galbraith,H.;Dempster,D.G.;Miller,T.B.
  4. Acta Endocrinol Suppl v.271 Deca-durabolin and parenteral nutrition in post-traumatic patients Mosebach,K.O.;Hausmann,D.;Caspari,R.;Stoeckel,H.
  5. Br J Surg v.50 The effect of methandienone on the metabolic response to surgical operation Johnston,G.R.;Chenneour,R.
  6. J Trauma v.22 Effect of an anabolic steroid on nitrogen balance and amino acid patterns after total hip replacement Michelsen,C.B.;Askanazi,J.;Kinney,J.M.;Gump,F.E.;Elwyn,D.H.
  7. Br J Clin Pract v.27 The effect of an anabolic steroid on postoperative nitrogen balance Tweedle,D.;Walton,C.;Johnston,I.D.A.
  8. Ann Neurol v.20 Effect of testosterone on muscle protein synthesis in myotonic dystrophy Griggs,R.C.;Halliday,D.;Kingston,W.;Moxley,R.T.
  9. Am J Physiol v.229 Interaction of anabolic steroids with glucocorticoid receptor sites in rat muscle cytosol Mayer,M.;Rosen,F.
  10. J Appl Physiol v.63 no.1 Testosterone fails to prevent skeletal muscle atrophy from glucocorticoids Capaccio,J.A.;Kurowski,T.T.;Czerwinski,S.M.;Chatterton Jr,R.T.;Hickson,R.C.
  11. Biochem J v.178 Effect of glucocorticoid administration on the rate of muscle protein breakdown in vivo in rats, as measured by urinary excretion of 3-methylhistidine Tomas,F.M.;Munro,H.;Young,V.R.
  12. Biochem J v.192 A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [³H]phenylalanine Garlick,P.J.;McNurlan,M.A.;Preedy,V.R.
  13. J Biol Chem v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;Rosebrough,N.J.;Farr,A.L.;Randall,R.J.
  14. Mammalian protein metabolism v.Ⅲ Analysis of tissues and body fluids for nitrogenous constituents Munro,H.;Fleck,A.;Munro,H.(ed.)
  15. Anal Biochem v.134 Improved liquid chromatographic determination of serum cortisol with double internal strandardisation compared to radioimmnoassay and fluorimetry, and evaluation by isotope dilution/mass spectrometry Lambert,W.E.;DeSlypere,J.M.;Jonchheere,J.A.;Vermeulen,A.;DeLeenheer,A.P.
  16. Anal Biochem v.108 Determination of prednisolone, prednisone, and cortisol in human plasma by high-performance liquid chrmatography Scott,N.R.;Chakraborty,J.;Marks,V.
  17. Br J Clin Pract v.30 no.1 Mode of action and use of anabolic steroids Gribbin,H.R.;Matts,S.G.F.
  18. Biochem J v.214 Time course of the effect of catabolic doses of corticosterone on protein turnover in rat skeletal muscle and liver Odedra,B.R.;Bates,P.C.;Millward,D.J.
  19. Aust J Agric Res v.31 Relationships of circulating cortisol levels with growth rate and meat tenderness of cattle and sheep Purchas,R.W.;Barton,R.A.;Kirton,A.H.
  20. J Anim Sci v.46 Relationships of some endocrine measurements to growth and carcass composition of cattle Trenkle,A.;Topel,D.G.
  21. Diabetes/Metab Rev v.4 Endocrine regulation of protein breakdown in skeletal muscle Kettelhut,I.C.;Wing,S.S.;Goldberg,A.J.
  22. Biochem J v.204 Effect of corticosterone treatment on muscle protein turnover in adrenalectomised rats and diabetic rats maintained on insulin Odedra,B.R.;Millward,D.J.
  23. Am J Physiol v.264 Glucocorticoids activate the ATP-ubiquitine-dependent proteolytic system in skeletal muscle during fasting Wing,S.S.;Goldberg,A.L.
  24. The control of fat and lean deposition Hormonal regulation of muscle protein synthesis and degradation Reeds,P.J.;Davis,T.A.;Buttery,P.J.(ed.);Boorman,K.N.(ed.);Lindsay,D.B.(ed.)
  25. Protein turnover in mammalian tissues and in the whole body Waterlow,J.C.;Garlick,P.J.;Millward,D.J.
  26. J Endocr v.114 Effects of the anabolic steroid stanozolol on growth and protein mrtabolism in the rat Bates,P.C.;Chew L.F.;Millward,D.J.
  27. Substrate and energy metabolism Reduction of corticosterone induced skeletal muscle protein catabolism by testosterone propionate Hayashi,K.;Kayali,A.;Moldawer,L.L.;Young,V.R.;Garrow,J.S.(ed.);Halliday,D.(ed.)
  28. J Anim Sci v.53 Androgen and glucocorticoid receptors in porcine skeletal muscle Snochowski,M.;Lundstrom,K.;Dahlberg,E.;Petersson,H.;Edquist,L.E.
  29. Endocrinology v.73 Pituitary-adrenal function in the rat after gonadectomy and gonadal hormone replacement Kitay,J.I.
  30. J Endocr v.25 Effect of an anabolic steroid methandienone on the metabolism of cortisol in the human James,V.H.T.;Landon,J.;Wynn,V.