Production of Inulin Fructotransferase(depolymerizing) from Flavobacterium sp. LC-413

  • Cho, Chul-Man ;
  • Lim, Young-Soon ;
  • Kang, Soo-Kyung ;
  • Jang, Kyung-Lib ;
  • Lee, Tae-Ho
  • Published : 1996.06.01


A bacterial strain LC-413, producing extracellular inulin fructotransferase which converts inulin into di-D-fructofuranose dianhydride(DFAIII) and amount of oilgosaccharides, was isolated from soil and pre-sumed as Flavobacteium sp. LC-413. The enzyme production was induced by inulin as carbon source and enhanced by the addition of 0.3% malt extract and 0.2% {TEX}$NaNO_{3}${/TEX} as nitrogen source. The enzyme activity in the culture supernatant reached at the maximum, 78.6units/ml, after 11 hours of cultivation in the medium composition of 1.5% inulin, 0.2% {TEX}$NaNO_{3}${/TEX}, 0.05% {TEX}$K_{2}${/TEX}{TEX}$HPO_{4}${/TEX}, 0.05% {TEX}$MgSO_{4}${/TEX}.7{TEX}$H_{2}${/TEX}O, 0.05% KCI, a trace amount of {TEX}$FeSO_{4}${/TEX}.7{TEX}$H_{2}${/TEX}O, and 0.3% malt ext. at 3$0^{\circ}C$. The oilgosaccharide produced by enzyme reaction from inulin was identified as DFA III by and {TEX}${13}^C${/TEX}-NMR spectrosocpy.


Flabobacterium sp. LC-413;inulin fructotransferase;DFA III


  1. J. Ferment. Technol. v.66 Inulinase from Chrysoporium pannorum Xiao,R.;Tanida,M.;Takao,S.
  2. Nippon Nogeikagaku Kaishi. v.52 Crystallization and general properties of an extracellular inulinase from Aspergillus sp Nakamura,T.;Kurokawa,T.;Nakatsu,S.;Ueda,S.
  3. Biochim. Biophys. Acta v.284 Formation of di-D-fructofuranose 1,2' : 2.3' dianhydride from inulin by an extracellular inulinase of Arthrobacter ureafaciens Tanaka,K.;Uchiyama,T.;Ito,A.
  4. Biochim. Biophys. Acta v.57 Purification and chracterization of Di-D-fructofuranose 1,2' : 2,1'-dianhydride producing enzyme from Streptomyces sp. MCI-2524 Cushibe,S.;Sashida,R.;Morimoto,Y.;Ohkishi,H.
  5. Kor. J. Micrbiol. Biotechnol. v.21 Production of inulin fructotransferase(depolymerizing) from Enterobacter sp. S45 Kang,S.I.;Kim,S.I.
  6. J. Ferment. Bioeng. v.72 Production of inulin fructotransferase(depolymerizing) from Arthrobacter sp. H65-7 and preparation of DFAⅢ from inulin by the enzyme Yokota,A.;Hirayama,S.;Enomoto,K.;Miura,Y.;Takao,S.;Tomita,F.
  7. Appl. Biochem. Biotechnol. v.34 Fructose and fructose-anhydrides from Dahlia inulin Hauly,M.C.O.;Bracht,A.;Beck,R.;Fontana,J.D.
  8. Bergy's manual of systematic bacteriology v.1 Krig,N.R.
  9. J. Biochem. v.90 Enzymatic formation of difructose anhydride Ⅳ from bacterial levan Tanaka,K.;Kawaguchi,H.;Ohno,K.;Shohji,K.
  10. Carbohydr. Res. v.101 Anomeric configurations of di-D-fructose anhydrideⅢ produced by inulin D-fructotransferase Uchiyama,T.
  11. Agric. Biol. Chem. v.55 Isolation and identification of the Aspergillus fumigatus difructose anhydride Matsuyainn,T.;Tanaka,K.;Uchiyama,T.
  12. Adv. Appl. Microbiol. v.29 Microbial inulinase : Fermentation process, properties, and applications Vandamme,E.J.;Derycke,D.G.
  13. J. Biol. Chem. v.178 A photometric method for the determination of inulin in plasma and urine Roe,J.H.;Epstein,J.H.;Goldstein,H.P.