Preventive Effect of Ecklonia Stolonifera on the Frequency of Benzo(a)pyrene-Induced Chromosomal Aberrations

  • Lee, Ji-Hyeon ;
  • Hye- Young Oh ;
  • Park, Jae-Sue
  • Published : 1996.06.01

Abstract

Chromosomal aberration tests in vitro using Chinese hamster lung(CHL) cells were carried out to evaluate the possible role of the MeOH extract of Ecklonia stolonifera in modulating the chromosomal damage induced by Mitomycin C(MMC) and Benzo(a)pyrene(B(a)P), respectively. The MeOH extract of Ecklonia stolonifera(260$\mu\textrm{g}$/ml) reduced significantly the incidence of chromosomal aberration induced by treatment with B(a)P by 80%. The suppressive effect was much stronger than that of $\beta$-carotene, which is well known antimu-tagen. However, there was no marked decrease in the chromosomal aberration induced by MMC. In the tests involving chromosomal aberration induced by the treatment of the MeOH extract of Exklonia stlolnifera alone, there was no significant increase in comparison with the negative control. The results would seem to indicate that. at least under the conditions examined, the MeOH extract of Ecklonis stolonifera decreased the chromosomal aberrations induced by B(a)P in the CHL cells, but had little effect on the chromosomal aberration induced by MMC.

Keywords

Ecklonia Stolonifera;marine algae;anti-chromosomal aberration;benzo(a)pyrene

References

  1. Bull. Koresn Fish. Soc. v.23 Antitumor effect and immunology activity of seaweeds toward Sarcoma-180 Cho,K.J.;Lee,Y.S.;Ryu,B.H.
  2. J. Korean Cancer Association v.23 Antitumor effects of selected medicinal plant components to implanted Sarcoma 180 in the mouse Hah,J.C.;Choe,E.S.;Rhew,T.H.;Young,H.S.;Park,K.Y.
  3. Nippon Suisan Gakkaishi v.55 Studies on the antitumor activity of marine algae Hiroyuki,N.;Hideomi,A.;Koichi,A.;Shinziro,H.;Kazutosi,N.
  4. Nippon Suisan Gakkaishi v.55 Antitumor activity of polysaccharides and lipids from marine algae Hiroyuki,N.;Hideomi,A.;Koichi,A.;Shinziro,H.;Kazutosi,N.
  5. Japan J. Exp. Med. v.44 Antitumor effect of extracts from Sargassum and Laminaria Yamamoto,I.;Nagumo,T.;Yagi,K.;Tominaga,H.;Aoki,M.
  6. Kitasato Arch. Exp. Med. v.61 Separation of sulfated, fucose-containing polysaccharides from the brown seaweed Sargassum kjellmanianum and their heterogeneity and antitumor activity Terukazu,N.;Noriko,I.;Michio,F.;Junko,H.;Kanki,K.;Iwao,U.
  7. Kor. J. Pharmacogn. v.24 Screening for antioxidant activity of plants and marine algae and its active principles from Prunus davidiana Choi,J.S.;Lee,J.H.;Kim,H.G.;Young,H.S.;Mun,S.I.
  8. Arch. Pharm. Res. v.19 The antioxidant activity of Ecklonia stolonifera Lee,J.H.;Park,J.C.;Choi,J.S.
  9. Mut. Res. v.323 Antioxidative and antimutagenic effects of theaflavins from blacktea Shiraki,M.;Hara,Y.;Osawa,T.;Kumon,H.;Nakayama,T.;Kawakishi,S.
  10. Chemistry Lettes Ecklonialactone-A and -B, two unusual metabolites from the brown algae Ecklonia stolonifera Okamura Kurata,K.;Taniguchi,K.;Shraishi,K.;Hayama,N.;Tanaka,I.;Suzuki,M.
  11. Phytochemistry v.33 Ecklonialactones-C-F from the brown alga Ecklonia stolonifera Kurata,K.;Taniguchi,K.;Shiraishi,K.;Suzuki,M.
  12. Nippon Suisan Gakkaishi v.57 Feeding-deterrent effect of phlorotannins from the brown alga Ecklonia stolonifera against the abalone Hahotis discus hannai Taniguchi,K.;Kurata,K.;Suzuki,M.
  13. Nippon Suisan Gakkaishi v.58 Feeding-deterrent activity of some Laminanaceous brown algae against the Ezo-abalone Taniguchi,K.;Kurata,K.;Suzuki,M.
  14. Progerss in Mutation Research v.5 The in vitro chromosomal aberration test using Chinese hamster lung(CHL) cells in culture Ishidate,M.;Sofuni,T.
  15. Environmental Mutagens & Carcinogens v.14 Evaluaton of the genetic toxicity of synthetic chemicals(Ⅰ). Chromosome aberration test on Chinese hamster lung cells in vitro Ryu,J.C.;Lee,S.Y.;Kim,K.R.;Park,J.S.
  16. Proc. Natl. Acad. Sci. v.73 Enzymatic conversion of benzo(a)pyrene leading predominantly to the diol epoxide through a single enantiomer Yang,S.K.;McCourt,D.W.;Roller,P.P.;Gelboin,H.V.
  17. Nature v.252 Metabolic activation of benzo(a)pyrene proceeds by a diol-epoxide Sims,P.;Grover,P.P.;Swaisland,A.;Pal,K.;Hewer,A.
  18. Proc. Natl. Acad. Sci. v.79 Mechanism of the inhibition of mutagenicity of a benzo(a)pyrene 7,8-diol-9,10-epoxide by riboflavin 5'-phosphate Wood,A.W.;Sayer,J.M.;Newmark,H.L.;Yagi,H.;Michaud,D.P.;Jerina,D.M.;Conney,A.H.
  19. Carcinogenesis v.4 Inhibition of the mutagenicity of bay region diol-epoxides of polycyclic aromatic hydrocarbons by phenolic plant flavonoids Huang,M.T.;Wood,A.W.;Newmark,H.L.;Sayer,J.M.;Yagi,H.;Jerina,D.M.;Conney,A.H.
  20. J. Korean Soc. Food Nutr. v.21 Antitumor and immunomodulating effects of seaweeds toward Sarcoma-180 cell Lee,Y.S.;Kim,D.S.;Ryu,B.H.;Lee,S.H.
  21. Carbohydate Research v.125 Purification and chemical and physical characterization of an antitumor polysaccharide from the brown seaweed Sargassum fulvellum Michio,F.;Noriko,I.;Yamamoto,I.;Nagumo,T.