Ascorbic Acid와 Pyrophosphate로부터 Ascorbic Acid-2-Phosphate의 효소적 생산

  • 최현일 (고려대학교 자연자원대학 농화학과) ;
  • 이상협 (고려대학교 자연자원대학 농화학과) ;
  • 방원기 (고려대학교 자연자원대학 농화학과)
  • Published : 1996.10.01


Microorganisms capable of producing ascorbic acid-2-phosphate (AsA2P) from ascorbic acid (AsA) and pyrophosphate (PPi) were screened from the culture collection of this laboratory. Among them, Cellulomonas sp. AP-7 showed the highest productivity of AsA2P. The optimal conditions for the production of AsA2P from AsA and PPi with cell-free extract as an enzyme source were investigated. The reaction mixture for the maximal production of AsA2P consisted of 21 g protein of cell-free extract per liter as the enzyme source, 250 mM AsA, 200 mM sodium pyrophosphate, 150 mM sodium acetate buffer (pH 4.5). By using this reaction mixture, 31.9 mM of AsA2P, which corresponded to a 12.76% yield based on AsA, was produced after incubation of 48 hr at 33$\circ$C.



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