Changes in activities of protease, phenoloxidase and cellulase during mycelium growth of Pleurotus ostreatus in sawdust cultures

톱밥배양한 느타리버섯 균사생장시 생산되는 각종 효소변화

  • Chang, Hyun-You (Division of Applied Microbiology, Agricultural Sciences and Technology Institute, RDA) ;
  • Kim, Gwang-Po (Division of Applied Microbiology, Agricultural Sciences and Technology Institute, RDA) ;
  • Cha, Dong-Yeul (Division of Applied Microbiology, Agricultural Sciences and Technology Institute, RDA)
  • 장현유 (농업과학기술원 응용미생물과) ;
  • 김광포 (농업과학기술원 응용미생물과) ;
  • 차동열 (농업과학기술원 응용미생물과)
  • Published : 1996.06.30


Effects of various kinds of sawdusts, supplements and culture conditions on activities of several enzymes such as protease, phenoloxidase and cellulase produced from mycelium of P. ostreatus grown on sawdust medium were studied and the results are as follows; Higher specific activity of these enzymes was observed when oak tree sawdust and poplar tree sawdust were supplemented with rice bran or wheat bran at rate of 30%, 20% and 10% in total volume respectively. Higher total activities of protease, phenoloxidase and cellulase were observed at 70% of the moisture contents of culture media, while lower activity of these enzymes was observed with 40% moisture contents of sawdust culture medium. The pH 4 and 9 of the sawdust media appeared to be optimum pH for the. production of protease while pH 5 and 7 were optimal for the production of phenoloxidase. The pH 6 of the sawdust medium was optimal for the production of cellulase. The optimum incubating temperature for the production of protease, phenoloxidase and cellulase was $25^{\circ}C$. Higher total activities of protease and phenoloxidase were observed when culture medium was added with wood vinegar at the control, and 0.5% for cellulase.


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