Comparative Induction of Sex-linked lethal mutations in Drosophila melanogaster males by Ethyl methanesulfonate

Drosophila meianogaster에서 Ethyl methanesulfonate에 의한 반성 치사 돌연변이 유발성의 비교

  • Choi, Yung-Hyun (Dept. of Biology, College of Natural Science, Pusan National University) ;
  • Yoo, Mi-Ae (Dept. of Molecular Biology, College of Natural Science, Pusan National University) ;
  • Lee, Won-Ho (Dept. of Biology, College of Natural Science, Pusan National University)
  • 최영현 (부산대학교 자연과학대학 생물학과) ;
  • 유미애 (부산대학교 자연과학대학 분자생물학과) ;
  • 이원호 (부산대학교 자연과학대학 생물학과)
  • Published : 1995.12.01


In order to analyze the sex-linked lethal mutagenic effects of ethyl methanesulfonate (EMS) in Drosophila melanogaster, the mutagenicities from the attached-X and Basc method have been detected. The indexes of relative lethal mutations at 2.0 and 4.0mM EMS treated group using the attached-X method were about 1.5 and 2.4 times than that of 1.0mM treated group, respectively. EMS had more pronounced effect in the sperm and spermatid stages in the induction of X-linked lethal mutations during the spermatogenesis. And, in the induction of X-linked recessive lethal mutations from the Basc method, the mutation frequency of 4.0mM EMS treated group as compared with 2.0mM was more than three fold. Two assay systems used in this study can support mutually according to experimental purposes, and the area of its application can be considerably wide.


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