The Effect of Plant Hormones and Light Quality on the Invertase Activity in Maize (Zea mays L.) and Mung Bean (Phaseolus radiatus L.)

옥수수와 녹두의 Invertase Isozymes 활성에 미치는 식물호르몬 및 광선의 효과

  • Lee, Dong-Hee (Dept. of Biology, College of Natural Sciences, Pusan National University) ;
  • Hong, Jung-Hee (Dept. of Biology, College of Natural Sciences, Pusan National University) ;
  • Kim, Young-Sang (Dept. of Biology, College of Natural Sciences, Pusan National University)
  • 이동희 (부산대학교 자연과학대학 생물학과) ;
  • 홍정희 (부산대학교 자연과학대학 생물학과) ;
  • 김영상 (부산대학교 자연과학대학 생물학과)
  • Published : 1995.10.01

Abstract

The effects of plant hormones (NAA, $GA_3$ and BA) and light qualities (white, red, green and blue light) on the changes of reducing sugar contents and invertase isozyme activities in leaves of maize (Zea mars L.) and mung bean (Phseolw radiatus L.) seedlings were investigated. NAA accelerated the increase of reducing sugar contents and invertase isozyme activities, on the contrary, $GA_3$ had little effect in the accumulation of reducing sugar and in the increase of enzyme activities from the leaves of maize and mung bean seedlings. On the other hand, BA accelerated an increase in the activities of the invertase isozyme from the leaves of mung bean seedlings whereas it had little effect in the increase of the enzyme activities from those of maize seedlings. The accumulation of reducing sugar in leaves of both seedlings was promoted by red light irradiation compared to white light irradiation, while the activities of the enzyme were little affected by various light Qualities. In the simultaneous applications of plant hormone and light quality, NAA with white light was very effective in the increase of reducing sugar contents and the enzyme activities from the leaves of mung bean seedlings, whereas NAA application with blue light showed a prominent enhancement in the reducing sugar contents and the enzyme activities from those of maize seedlings. These results suggest that plant hormone, particularly NAA, may be a more important factor than various light Qualities in the stimulation of invertase activity.

Keywords

acid invertase;alkaline invertase;maize;mung bean;NAA;GA3;BA;light quality

References

  1. Plant Physiol. v.73 Invertase activity and the kinetinstimulated enlargement of detached radish cotyledons Howard,H.F.;F.H.Witham
  2. J. Biol. Chem. v.135 A photometric adaptation of the Somogyi method for the determination of glucose Nelson,M.F.
  3. Phytochemistry v.12 Distribution in excised Lycopersicum esculentum roots of the principal enzyme involved in sucrose metabolism Chin,C.K.;G.D.Weston
  4. Plant Physiol. v.43 Promotion of growth and invertase activity by gibberellic acid in developing Avena internodes Kaufman,P.B.;N.Ghosheh;H.Ikuma
  5. Planta v.182 Impermeant auxin analogues have auxin activity Venis,M.A.;E.W.Thomas;H.Barbier-Brygo;G.Ephritikhine;J.Guern
  6. Methods of Enzymatic Analysis v.2 D-Glucose Bergmeyer,H.U.;E.Bernt;H.U.Bergmeyer(ed.)
  7. Storage Carbohydrates in Vascular Plants Sucrose metabolism ap-Rees,T.;D.H.Lewis(ed.)
  8. Plant Physiol. v.54 Changes in invertase activity of sweet potato in response to wounding and purification and properties of its invertases Matsushita,K.;I.Uritani
  9. Plant Physiol. v.91 Purification and characterization of soluble (cytosolic) and bound (cell wall) isoforms of invertases in barley (Hordeum vulgare) elongating stem tissue Karuppiah,N.;B.Vadlamudi;P.B.Kaufman
  10. Plant Physiol. v.65 Invertase in oat seedlings. Separation, properties and changes in activities in seedling segments Pressey,R.;J.Avants
  11. J. Plant Physiol. v.124 Influence of sucrose and hormones on the activity of sucrose synthase and invertase in detached leaves and leaf sections of eggplants (Solanum melongena) Claussen,W.;B.R.Loveys;J.S.Hawker
  12. Signal perception and transduction in higher plants The sensitivity of plant protoplasts to auxin is likely modulated by the number of receptors at the plasmalemma Barbier-Brygo,H.;G.Ephritikhine;D.Kiambt;J.Guern;R.Ranjeva(ed.);A.M.Boudet(ed.)
  13. Archives of Biochemistry and Biophysics v.296 Biochemical and immunological properties of alkaline invertase isolated from sprouting soybean hypocotyls Chen,J.G.;C.C.Black
  14. J. Korean Environ. Sci. Soc. v.2 no.4 The effect of plant hormones and light quality on the formation of chlorophyll-protein complexes in maize seedlings Lee,D.H.;Y.S.Kim
  15. Physiol. Plant v.65 Effects of gibberellic acid on patterns of carbohydrate distribution and acid invertase activity in Phaseolus vulgaris Morris,D.A.;E.D.Arthur
  16. Biochem. J. v.178 The location of acid invertase activity and sucrose in the vacuoles of storage root of beet root (Beta vulgaris) Leigh,R.A.;T.ap-Rees;W.A.Fuller;J.Banfield
  17. Plant Physiol. v.72 Pathway of phloem unloading of sucrose in corn roots Giaquinta,R.T.;W.Lin;N.L.Sadler;V.R.Franceschi
  18. Physiol. Plant v.84 Sugar accumulation in growing subhooks of etiolated Pisum sativum seedlings-Stimulation of sugar exudation and invertase activity in epicotyls by gibberellic acid Miyamoto,K.;J.Ueda;S.Kamisaka
  19. Phytochemistry v.23 Invertase and auxin-induced elongation in internodal segments of Phaseolus vulgaris Morris,D.A.;E.D.Arthur
  20. Plant Cell v.2 cDNA cloning of carrot extracellular β-fructosidase and its expression in response to wounding and bacterial infection Sturm,A.;M.J.Chrispeels
  21. Korean J. Bot. v.35 no.1 Changes of sucrose content and invertase activity in leaves of barley seedlings under low temperature Lee,M.A.;J.S.Maeng;S.G.Kim;Y.M.Kwon
  22. Plant physiol. v.56 Promotion of radish cotyledon enlargement and reducing sugar content by zeatin and red light Huff,A.;C.Ross
  23. Acta. Horticul. v.226 Vegetative propagation of Syringa vulgaris L. in vitro Pierik,R.L.M.;H.H.M.Steegmans;A.A.Elias;O.T.J.Stiskema;A. J. van der Velde