Detection of the Specific DNA-binding Proteins for the Aphid rRNA

진딧물 rRNA 유전장에 특이적으로 결합하는 단백질 탐색

  • O-Yu Kwon (Division of Endocrinology & Metabolism, Beth Israel Hospital/Harvard Medical School) ;
  • Dong-Hee Lee (Division of Endocrinology & Metabolism, Beth Israel Hospital/Harvard Medical School) ;
  • Tae-Young Kwon (Kyngpook Provincial Rural Development Administration)
  • Published : 1995.06.01

Abstract

A whole body extract (WBE), a crude nuclear fraction, of aphids was prepared and used to identify the proteins which bound specifically to 5'-upstream regions of the transcription initiation site of the aphid ribosomal RNA gene (rDNA). While DNA fragment (-263/-195) was bound by only one specific 53 kDa protein, two DNA fragments, A(-194/23) and B(-393/-264), were commonly bound by three proteins (52 kDa, 50 kDa and 40 kDa). It was also revealed that the formation of he DNA-protein complex requires a cation.