Production of Inulin fructotransferase(depolumerizing)by Arthrobacter sp. A-6

Arthrobacter sp. A-6에 의한 Inulin Fructotransferase (depolymerizing)의 생산

  • 박정복 (고려대학교 자연자원대학 유전공학과) ;
  • 권영만 (고려대학교 자연자원대학 유전공학과) ;
  • 최용진 (고려대학교 자연자원대학 유전공학과)
  • Published : 1995.02.01


A bacterial strain A-6 producing the high level of an extracellular inulin fructotransfe rase(depolymerizing)(EC which converts inulin into di-D-fructofuranose dianhydride III (DFAIII) was isolated from soil. The isolated strain could be classified as a species belonging to the genus Arthrobacter based on its morphological and physiological characteristics identified in this work. Production of the enzyme was induced by inulin, and the highest activity was detected in the slightly acidic medium supplemented with 2.5% inulin and 0.1% trypton as a sole carbon and a nitrogen source, respectively. Under the optimal conditions, the enzyme activity in the culture supernatant reached approximately 60 uints/ml after 96 hours of cultivation. The optimum pH and temperature for the crude enzyme preparation from Arthrobacter sp. A-6 were pH 5.0 and 60$\circ$C , respectively. The DFA produced by the action of the inulin fructotransferase was confirmed to be DFAIII by paper chromatography, HPLC and $^{13}$C-NMR spectroscopy.


  1. Jpn. Kokai Tokkyo Koho JP 03 67,560 (9167,560)(CI.A23LI/236) Sweetering compositions containing di-D-fructofuranose 1,2′:2,3′ dianhydride and menthol K.Tsutomu;N.Akio
  2. Kor. J. Microbiol. Biotechnol. v.21 no.1 Enterobacter sp. S45에 의한 inulin fructotransferase의 생산 강수일;김수일
  3. Nature. v.167 Improved spraying reagents for the detection of sugars on paper chromatograms J.L.Bryson;T.J.Mitchell
  4. J. Biochem. v.78 no.6 Enzymatic hydrolysis of di-D-fructofuranose 1,2′:2,3′ dianhydride with Arthrobacter ureafaciens Tanaka,T.;Y.Uchiyama;H.Kobori;T.Tanaka
  5. Jpn. Kokai Tokkyo Koho JP 63,269,962(88,269,962)(CI.A23LI/305) Food ingredients containing difructose dianhydrides K.Shoichi;S.Koji;H.Kazumoto;K.Mamoru;N.Kazushi;H.Keikichi;K.Keiji;Mitsuru
  6. Bergey's manual of Systematic Bacteriology v.1 Krieg,N.R.
  7. Agric. Biol. Chem. v.52 no.12 Purification and some properties of inulin fructotransferase (depolymerizing) from Arthrobacter ilicis Kawamura,M.;S.Takahashi;T.Uchiyama
  8. Biosci. Biotech. Biochem. v.58 no.6 Production of cyclofructan from inulin by Bacillus circulans MCI-2554 S.Kushibe;R.Sashida;Y.Morimoto
  9. Agric. Biol. Chem. v.53 no.8 Purification and properties of a novel inulin fructotransferase (DFAI-producing) from Arthrobacter globiformis S14-3 Seki,K.;K.Haraguchi;M.Kishimot;S.Kobayashi;K.Kainuma
  10. Biosci. Biotech. Biochem. v.58 no.3 Purification of inulin fructotransferase (DFAI-producing) from Arthrobacter sp. MCI 2493 and Production of DFAI from Inulin by the Enzyme M.Ueda;R.Sashida;Y.Morimoto;H.Ohkishi
  11. Agric. Biol. Chem. v.55 no.5 Isolation and identification of the Aspergillus fumigatus difructose dianhydride Matsuyama,T.;K.Tanaka;T.Uchiyama
  12. Jpn. Kokai Tokkyo Koho JP 05,168,419 /993,168,419)(CI.A23KI/16) Lipid-reducing agents containing difructose derivative and breeding animals using them K.Tosha;N.Akio
  13. American Society for Microbiology. Manual of Methods for General Bacteriology Gerhardt,P.;G.E.G.Murray;R.N.Gostilow;F.W.Nester;A.W.Wood;N.R.Krig;G.B.Philips
  14. Biochim. Biophys. Acta. v.284 Formation of di-D-fructofuranose 1,2 2,3 dianhydride from inulin by an extracellular inulinase of Arthrobacter ureafaciens Tanaka,K.;T.Uchiyama;A.Ito
  15. J. Biochem. v.92 Enzymatic formation of di-D-fructose-1,2′:2:1′-dianhydride from inulobiose by Aspergillus fumigatus Matsuyama,T.;K.Tanaka;M.Mashiko;M.Kamamoto
  16. J. Inst. Brew. v.84 Determination of total carbohydrate in beer Weiner,J.
  17. Journal of Fermert. Bioeng. v.72 no.4 Production of inulin fructotransferase (depolymerizing) by Arthrobacter sp. H65-7 and preparation of DFAIII from inulin by the enzyme A.Yokota;S.Hirayama;K.Enomoto;Y.Miura;S.Takao;F.Tomita
  18. Carbohydrate Res. v.101 Anomeric configurations of di-D-fructose anhydride, produced by fructotransferase Uchiyama,T.
  19. Agric. Biol. Chem. v.52 no.1 Purification and properties of inulin fructotransferase (depolymerizing) from Arthrobacter globiformis CII-I Haraguchi,K.;M.Kishimoto;K.Seki;K.Hayashi;S.Kobayashi;K.Kainuma
  20. Agric. Biol. Chem. v.53 no.3 On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose Matsuyama,T.;K.Tanaka