The Effects of Amino Acids and Metaolites on the Biosynthesis of Biodegradative Theronine Dehydratase in Serratia matcescens ATCC25419

아미노산과 대사산물들이 Serratia marcescens Biodegradative Threonine Dehydratase의 생합성에 미치는 영향

  • 최병범 (연세대학교 이과대학 생화학과) ;
  • 김승수 (연세대학교 이과대학 생화학과 생물산업소재연구센터)
  • Published : 1995.02.01

Abstract

The effects of amino acids in growth media on the biosynthesis of Serratia marcescens biodegradative threonine dehydratase activity were examined. The enzyme activity was decreased by 44 and 34% by 10 mM isoleucine and valine, respectively, whereas it was increased approximately by 20% by 10 mM threonine. Among several metabolites tested, pyruvate increased the enzyme activity by 60% at 5 mM, but decreased the enzyme activity approximately by 20 to 70% above 20 mM. The enzyme activity was increased by 64% by 5 mM glyoxylate, whereas it decreased the enzyme activity approximately by 40 to 70% above 20 mM glyoxylate. The thiamine, monopyrrole derivative, also increased the enzyme activity by 84% at 50 $\mu $g/ml, but did not affected the enzyme activity above 300 $\mu $g/ml. cAMP increased the enzyme activity by 58% at 0.5 mM, but decreased the enzyme activity by 15% at 2 mM. These data suggested that the biosynthesis of Serratia marcescens biodegradative threonine dehydratase is regulated by concentrations of pyruvate, glyoxylate and cAMP.

References

  1. Ann. Rev. Biochem. v.47 Amino acid Biosynthesis and its regulation Umbarger,H.E.
  2. J. Biol. Chem. v.245 Regulation of biodegradative threonine deaminase synthesis in Escherichia coli by cyclic adenosine 3´,5´-monophosphate Shizuta,Y.;O.Hayaishi
  3. J. Bacteriol. v.135 Control of biodegradative threonine dehydratase inducibility by cyclic AMP in energy-restricted Escherichia coli Phillips,A.T.;R.M.Egan;B.Lewis
  4. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;N.J.Rosenbrough;A.L.Farr;R.J.Randall
  5. Biochemistry. v.14 Catabolite inactivation of biodegradative threonine dehydratase of Escherichia coli Feldman,D.A.;P.Datta
  6. J. Biol. Chem. v.256 Mechanism of catabolite inactivation of Escherichia coli biodegradative threonine dehydratase by glyoxylate Park,L.S.;P.Datta
  7. J. Bacteriol v.114 Isoleucine and valine metabolism in Escherichia coli Umbarger,H.E.
  8. Curr. Top. Cell. Regul. v.11 Regulation of biodegradative threonine deaminase Shizuta,Y.;O.Hayaishi
  9. J. Bacteriol. v.114 Isoleucine and valine metabolism in Escherichia coli Umbarger,H.E.
  10. J. Bacteriol. v.132 Requirements for induction of the biodegradative threonine dehydratase in Escherichia coli Egan,R.M.;A.T.Phillips
  11. Biochemistry. v.17 Allosteric inhibition and catabolite inactivation of purified biodegradative threonine dehydratase of Salmonella typhimurium Bhadra,R.;P.Datta
  12. J. Bacteriol. v.132 Multivalent induction of biodegradative threonine deaminase Yui,Y.;Y.Watanabe;S.Ito;Y.Shizuta;O.Hayaishi
  13. J. Biol. Chem. v.181 Serine and threonine deaminases of Escherichia coli activators for a cell free enzyme Wood,W.A.;I.C.Gunsalus
  14. J. Biol. Chem. v.147 Pyruvic acid Ⅱ The determination of keto acids in blood and urine Friedemann,T.E.;G.E.Haugen
  15. Curr. Top. Cell. Regul. v.9 L-Threonine dehydratase as a model of allosteric control involving ligand-induced oligomerization Dunne,C.P.;W.A.Wood
  16. J. Bacteriol. v.60 Mutants of Escherichia coli requiring methionine or vitamin B Davis,B.D.;E.S.Mingioli
  17. J. Bacteriol. v.73 Threonine deamination in Escherichia coli. Ⅱ. Evidence for two L-threonine deaminases Umbarger,H.E.;B.Brown
  18. J. Bacteriol. v.155 Synthesis of biodegradative threonine dehydratase in Escherichia coli: role of amino acids, electron acceptors, and certain intermediary metabolites Hobert,E.H.;P.Datta