A Study of Protein Ion Exchange Chromatography based on Plate Theory

단이론에 따른 단백질 이온교환 크로마토그라피의 연구

  • 김인호 (충남대학교 화학공학과) ;
  • 김진태 (생명공학연구소 생물공정연구그룹)
  • Published : 1995.08.01

Abstract

Protein ion exchange chromatography was studied experimentally in order to prove the theoretical prediction from the linear model of Yamamoto, S. et al (1). Adsorption isotherms were measured as a function of ionic strength in a batch experiment. The relationship between the characteristics of chromatogram and the operating conditions of ionic strength, flow rate, length of column, concentration and amount of protein sample were studied. At the higher ionic strength, the lower flow rate and the longer column conditions, the higher number of plate was obtained. Satisfactory agreement was observed between the experimental and the calculated chromatograms except for the case of high protein concentration.

Keywords

Plate theory;protein ion exchange chromatograph

References

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