Identification and Culture Conditon of an Actionomycetes Stranin Producing an Angiotensin Converting Enzyme Inhibitor

Angiotensin Converting Enzyme(ACE) 저해제를 생성하는 방선균 분리주의 동정 및 최적 발효조건

  • Moon, Seong-Hoon ;
  • Ha, Sang-Chul ;
  • Lee, Dong-Sun ;
  • Kim, Jong-Guk ;
  • Hong, Soon-Duck
  • 문성훈 (경북대학교 미생물학과) ;
  • 하상철 (한국과학기술연구원 부설 생명공학 연구소) ;
  • 이동선 (경북대학교 미생물학과) ;
  • 김종국 (경북대학교 미생물학과) ;
  • 홍순덕 (경북대학교 미생물학과)
  • Published : 1995.08.01

Abstract

Identification of Actinomycetes isolate strain SH-8002, a producer of ACE inhibitor, based on procedures employed in the international Streptomyces project. The strain, designated as SH-8002, was identified as Streptomyces zoamyceticus SH-8002 based on its morphological, physiological, biochemical and chemotaxonomic characteristics. The ACE inhibitor produced by the strain was highly achieved in fermentation medium condition that was 1% soluble starch, 0.5% tryptone, 0.2% K$_{2}$HPO$_{4}$, 0.2% CaCO$_{3}$, 0.1% NaCl, pH 8.0 at 30$\circ$C for 144 hrs.

Keywords

Angiotensin converting enzyme inhibitor;identification;Strptomyces zoamyceticus SH-8002;optimum fermentation condition

References

  1. Chemical methods in Bacterial Systematics M.Goodfellow;David,E.M.
  2. J. Ferment. Technol. v.65 Humic acid-vitamin agar, a new medium for the selective isolation of soil Actinomycetes Masayuki,H.;H.Nonomura
  3. Med. Res. Rev. v.3 ACE inhibitors, Medicinal chemistry and biological actions Petrillo,E.W.;Ondetti,M.A.
  4. Laboratory method in Microbiology Harrigan,W.F.;M.E.McCance
  5. Bergey's Mannual of systemic bacterology.(4 ed.) Williams,S.T.(et al.)
  6. 한국생약학회지 v.12 식물생약의 안지오텐신 변환효소 억제 작용 검색 윤혜숙;정성현;한병훈
  7. J. Gen. Microbiol. v.129 Numerical classification of Streptomyces and related genera Williams,S.T.;M.Goodfellow;G.Alderson
  8. 강원대 대학원 발효공학과 공학박사 학위 논문 Angiotensin converting enzyme 저해제의 합성 및 동력학적 연구 이기호
  9. 약학회지 v.28;31 안지오텐신 변환효소 억제작용 물질의 합성 윤혜숙;이희주(외)
  10. J. Antibiotics v.39 K4. Novel inhibitor of angiotensin Ⅰ converting enzyme produced by Actinomadura spiculosospora Koguchi,T.;Yamada,K.;Kase,H.
  11. Int. J. Syst. Bacteriol. v.20 Chemical composition as a criterion in the classification of aerobic Actinomycetes Lechevalier,M.P.;H.Lecheavalier
  12. Methods in microbiology v.4 Booth,C.
  13. Int. J. Syst. Bacteriol. v.16 Methods for characterization of Streptomyces species Shirling,E.B.;D.Gottlieb
  14. J. Gen. Microbiol. v.129 A probability matrix for identification of some Streptomyces Williams,S.T.;M.Goodfellow;E.M.H.Wellihgton
  15. Anal. Biochem. v.84 A rapid and simple spectrophotomeric assay of angiotensin converting enzyme Hayakar,M.;Y.konod;H.Izumi
  16. J. Bacteriol. v.56 The utilization of carbon compounds by some Actinomycetes as an aid for species determination Gottlieb,D.;Pridham,T.G.
  17. Ann. Rev. Biochem. v.51 Enzyme of the renin-angiotensin system and their inhibitors Ondetti,M.A.;D.W.Cushman