Pufification of Cextranase by Aspergillus ustus GR-98

Aspergillus ustus GR-98이 생산하는 Dextranase의 정제

  • Lee, Jong-Tae ;
  • Do, Jae-Ho ;
  • Yang, Jae-Won ;
  • Kim, Chan-Jo
  • 이종태 (한국인삼연초연구원 제품개발부) ;
  • 도재호 (한국인삼연초연구원 제품개발부) ;
  • 양재원 (한국인삼연초연구원 제품개발부) ;
  • 김찬조 (충남대학교 식품공학과)
  • Published : 1995.08.01


The dextranase (EC produced by Aspergillus ustus GR-98 was purified by the following sequential methods; salting-out and dialysis, gel filtration on BIO-GEL P-100, ion exchange chromatography on DEAH-cellulose, affinity chromatography on hydroxyapatite, and preparative electrophoresis. Three active fractions, dextranases 1, 11 and 111, were isolated in electrophoretically pure states, and specific activities of the dextranases were 1,276, 1,154 and 1,125 units/mg, the degrees of yield were 9.0, 3.6 and 2.2%, having 145, 131.1 and 127.8 times as those of culture filtrate in degree of purification, respectively. The enzyme purity was confirmed by the PAGE, SDS-PAGE and get permeation-HPLC.


Aspergillus ustus GR-98;dextranase;purification


  1. 日本農藝化學會誌 v.57 デキストラナ-ゼ 溱貞正
  2. J. Biochem. v.69 Studies on mold dextranases, Ⅰ. Penicillium luteum dextranase: Its production and some enzymatic properties Fukumoto,J.;H.Tsuji;D.Tsuru
  3. Nippon Nogeikagaku Kaishi v.48 Some properties of the purified dextranase (Studies on the dextranase produced by Aspergillus ustus 237 Part Ⅱ) Hanada,K.;A.Hirose;E.Ninomiya
  4. J. Biochem. v.71 Studies on mold dextranases: Ⅲ. Purification and some enzymatic properties of Aspergillus carneus dextranase Hiraoka,N.;J.Fukumoto;D.Tsuru
  5. Biochem. J. v.91 Proteins by sephadex gel filtration Lowry,O.H.;N.J.Rosebrough;A.L.Farr;R.J.Randal
  6. Nature v.277 Cleavage of structure proteins during assembly of the head of bacteriophage T4 Laemmli,U.K.
  7. Infect. Immun. v.55 Purification and partial characterization of the multicomponent dextranase complex of Streptococcus sobrinus and cloning of the dextranase gene Barrett,J.F.;T.A.Barrett;R.Curtiss Ⅲ
  8. Eur. J. Biochem. v.183 The purification and characterization of a dextranase from Lipomyces starkey Koenig,D.;D.Day
  9. Appl. Microbiol. v.20 Isolation of a pure dextranase from Penicillium funiculosum Chaiet,L.;A.J.Kempt;R.Harman;E.Kaczka;R.Weston;K.Nollstadt;F.J.Wolf
  10. 한국균학회지 v.11 Aspergillus ustus가 생산하는 exo-dextranase의 정제에 관한 연구 이건주;이형환
  11. Enzyme Handbook v.2 Barman,T.E.
  12. Biochem. Biophys. Acta v.309 Studies on dextranase, Purification of dextranase from Penicillium funiculosum and its enzymatic properties Sugiura,M.;A.Ito;T.Ogiso;K.Kato;H.Asano
  13. Agric. Biol. Chem. v.45 The purification and characterization of the dextranase of Chaetomium gracile Hattori,A.;K.Ishibashi;S.Minato
  14. Dextranase 생산균주의 분리, 동정 및 효소 생산 이종태;이동희;곽이성;김영호;성현순;김찬조