Pufification of Cextranase by Aspergillus ustus GR-98

Aspergillus ustus GR-98이 생산하는 Dextranase의 정제

  • Lee, Jong-Tae ;
  • Do, Jae-Ho ;
  • Yang, Jae-Won ;
  • Kim, Chan-Jo
  • 이종태 (한국인삼연초연구원 제품개발부) ;
  • 도재호 (한국인삼연초연구원 제품개발부) ;
  • 양재원 (한국인삼연초연구원 제품개발부) ;
  • 김찬조 (충남대학교 식품공학과)
  • Published : 1995.08.01

Abstract

The dextranase (EC 3.2.1.11) produced by Aspergillus ustus GR-98 was purified by the following sequential methods; salting-out and dialysis, gel filtration on BIO-GEL P-100, ion exchange chromatography on DEAH-cellulose, affinity chromatography on hydroxyapatite, and preparative electrophoresis. Three active fractions, dextranases 1, 11 and 111, were isolated in electrophoretically pure states, and specific activities of the dextranases were 1,276, 1,154 and 1,125 units/mg, the degrees of yield were 9.0, 3.6 and 2.2%, having 145, 131.1 and 127.8 times as those of culture filtrate in degree of purification, respectively. The enzyme purity was confirmed by the PAGE, SDS-PAGE and get permeation-HPLC.

Keywords

Aspergillus ustus GR-98;dextranase;purification

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