Isolation and Identification of Dextranase Production Strains and Enzyme Production

Dextranase 생산균주의 분리, 동정 및 효소생산

  • Lee, Jong-Tae ;
  • Yi, Dong-Heui ;
  • Kwak, Yi-Seong ;
  • Kim, Young-Ho ;
  • Sung, Hyun-Soon ;
  • Kim, Chan-Jo
  • 이종태 (한국인삼연초연구원 제품개발부) ;
  • 이동희 (건국대학교 미생물공학과) ;
  • 곽이성 (한국인삼연초연구원 제품개발부) ;
  • 김영호 (한국인삼연초연구원 제품개발부) ;
  • 성현순 (한국인삼연초연구원 제품개발부) ;
  • 김찬조 (충남대학교 식품공학과)
  • Published : 1995.08.01

Abstract

In order to screen dextranase with high dextranolytic activity from microbial origins, dextranase producing fungal isolates were isolated from soil of the Taeion area. 197 strains with dextranolytic activities were isolated, out of which 3 strains with high dextranolytic activities were selected in the first screening. A strain (GR-98) with a best dextranolytic activity was selected in the second screening. The strain was identified to be similiar Aspergillus ustus by the morphological and cultural characteristics. The optimum culture temperature and initial pH for the dextranase production of the strain was 30$\circ$C and 7.0, respectively. The optimum culture medium was composed of 2% dextran, 0.3% KNO$_{3}$, 0.05% K$_{2}$HPO$_{4}$, 0.02% MgSO$_{4}$-7H$_{2}$O, 0.05% KC1, and 2.5 $\mu$g/ml pyridoxamine, and the enzyme production was maximum when the strain was subcultured at 30$\circ$C for 7 days.

Keywords

Dextranase;isolation and identification;Aspergillus ustus dextranase production

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