Introduction and Expression of the Urease Gene in Mosquitocidal Bacillus sphaericus 1593

세균성 Urease Gene에 의한 모기유충 방제균 Bacillus sphaericus 1593의 형질전환

  • 한길환 (영남대학교 응용미생물학과) ;
  • 김상달 (영남대학교 응용미생물학과)
  • Published : 1995.08.01

Abstract

Bacillus sphaericus 1593 is a larvicidal toxin-producing mosquitocidal bacterium. The toxin contains a parasporal crystalline inclusion which is composed of a protein that is activated under alkaline condition. To enhance alkaline environment around toxin protein, cryptic plasmid cured, B. sphaericus 1593 was transformed by the Bacillus pasteurii urease gene which generate ammonia from urea. Transformant produced urease at about 80% more than wild type strain. B. sphaericus 1593, and the urease gene was stably maintained. It also produced crystalline toxin protein at the same level as the wild type strain B. sphaericus 1593.

Keywords

Bacillus sphaericus 1593;mosquitocidal toxin;urease gene

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