Cloning and Sequencing of Resistance Determinants to Aminoglycoside Antibiotics from Sterptoalloteichus hindustanus ATCC 31219

Streptoalloteichus hindustanus ATCC 31219로부터 아미노글라이코사이드계 항생제에 내성을 지정하는 유전자의 클로닝 및 염기서열 결정

  • Kim, Jong-Woo ;
  • Han, Jae-Jin ;
  • Choi, Young-Nae ;
  • Eom, Joon-Ho ;
  • Yoon, Sung-Joon ;
  • Hyun, Chang-Gu ;
  • Suh, Joo-Won
  • 김종우 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 한재진 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 최영내 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 엄준호 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 윤성준 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 현창구 (명지대학교 생명과학연구소) ;
  • 서주원 (명지대학교 생명과학연구소)
  • Published : 1995.08.01

Abstract

Streptoalloteichus hindustanus ATCC 31219, a nebramycin complex producer, is similar to Streptomyeces tenebrarius in a viewpoint of resistance to a wide range of aminoglycoside antibiotics. S. tenebrarius has resistance mechanisms of 16s rRNA methylation and aminogycoside modification. However, it is not known whether resistance mechanisms of Stall. hindustanus are the same as in S. tenebrarius. Therefore, we have tried to isolate resistance determinants from Stall. hindustanus. Two different types of aminoglycoside resistance determinants were isolated from Stall. hindustanus and expressed in Streptomyces lividans TK24. The apramycin resistance gene (amr) and the tobramycin resistance gene (tmr) isolated from Stall. hindustanus showed broad resistance spectrum against a dozen of aminoglycoside antibiotics. The complete nucleotide sequences of apramycin resistance gene (amr) were determined. The deduced amino acid sequence of the amr gene of Stall hindustanus ATCC 31219 showed extensive sequence homology to the 16s rRNA methylase gene (kamB) of S. tenebrarius.

Keywords

Streptoalloteichus hindustanus ATCC 31219;resistance determinant;16S rRNA methylase;cloning homology

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