Survivals of Lactic Acid Bacteria and its Characteristics under the Acidic and Anaerobic Condition

혐기적 산성조건하에서 젖산균의 생존과 그 특성

  • 신용서 (원광대학교 농화학과) ;
  • 김성효 (원광대학교 농화학과) ;
  • 이갑상 (원광대학교 농화학과)
  • Published : 1995.08.01


We investigated the survival, $\beta$-galactosidase activity and cellular permeability of lactic acid bacteria such as Lactobacillus acidophilus ATCC 4356, Lactobacillus casei subsp. casei IFO 3533, Streptococcus thermophilus KCTC 2185, Lactobacillus delbrueckii subsp. lactis ATCC 4797, and Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 in anaerobic condition of pH 1.5-3.5 range. Numbers of all tested viable cells did not decrease at pH 3.5, but decreased rapidly at pH 1.5 and pH 2.5 during 2 hour incubation at modified EG medium. Immediately after 2 hour incubation, the decrease in population at pH 1.5 and pH 2.5 was about 6-8 and 5-7 log cycles/ml, respectively. Lactobacillus acidophilus ATCC 4356 showed the higest survival of all tested bacteria. The $\beta$-galactosidase activity from Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 and Streptococcus thermophilus KCTC 2185 decreased rapidly at pH 1.5 and 2.5, but there was a little decrease at pH 3.5. The cellular permeability that was measured by the leakage of intracellular materials increased with decrease of pH. These results suggest that the ingested lactic acid bacteria may be destroyed in contact with low pH of gastric acid.


Lactic acid bacteria;acidic and anaerobic condition;${\beta}$-galactosidase;acid-tolenrance;cellular permeabiliy


  1. CMA. J. v.105 Studies on natural gastric flora; survival of bacteria in fasting human subjects Franklin,M.A.;S.C.Skoryna
  2. Gut. v.16 Gastric pH and microflora of normal and diarrhoeic infants Maffei,H.V.L.;F.J.Norbrega
  3. Yogurt: Science and Technology Tamine,A.Y.;P.K.Robinson
  4. Appl. Environ. Microbiol. v.49 Assimilation of Cholesterol by Lactobacillus acidoplilus Gilliland,S.E.;C.R.Nelson;C.Maxwell
  5. J. Dairy Sci. v.59 Lactase activity of cultured and acidified dairy products Kilara,A.;K.M.Shahani
  6. Nature. v.224 Lactose Tolerance in Asians a Family Study Flatz,G.;C.Saengudom
  7. J. Dairy Sci. v.76 Influence of bile on cellular integrity and β-galactosidase activity of Lactobacillus acidophilus Noh,D.O.;S.E.Gilliland
  8. J. Food Sci. v.55 Survival of lactic acid bacteria and their lactase under acidic conditions Shah,N.;P.Jelen
  9. J. Dairy Sci. v.70 Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells Conway,P.L.;S.L.Gorbach;B.R.Goldin
  10. Am. J. Clin. Nutr. v.45 Lactose digestion by yogurt β-galactosidase: influence of pH and microbial cell integrity Martini,M.C.;G.L.Bollweg;M.D.Levitt;D.A.Savaiano
  11. Johns Hopkins Med. J. v.129 Lactose malabsorption and milk rejection in Negro children Paige,D.M.;T.M.Bayless;Ferry,G.D.
  12. Gut. v.13 Gastric acid barrier to ingested microorganisms in man; studies in vivo and in vitro Giannella,R.A.;S.A.Broitman;N.Zamchick
  13. 腸內 flora와 發癌(光岡之足編) 荒井辛一郞;室田一也;早川邦産;片岡元行;光岡之足
  14. Appl. Environ. Microbiol. v.53 Membrane ATPase and acid tolerance of Actinomyces viscosus and Lactobacillus casei Bender,G.R.;R.E.Marquis
  15. Cancer Res. v.35 The epidemiology of large bowel cancer Wynder,E.L.
  16. Microb. Rev. v.49 Regulation of cytoplasmic pH in bacteria Booth,J.R.
  17. Am. J. Clin. Natr. v.40 Lactose malabsorption from yogurt, pasteurized yogurt, sweet acidophilus milk, and cultured milk in lactose-deficient individuals Savaiano,D.A.;D.A.G.Abdelhak Abou Elanouar;Smith,D.E.;M.D.Levitt
  18. The lactic acid Bacteria in Health & Disease Wood,B.J.B.
  19. J. Food Sci. v.53 Effect of low pH on the ability of Lactobacillus acidophilus to survive and adhere to human intestinal cells Hood,S.K.;E.A.Zottola