Separation in Oxdized Mixture of Lipids and Phenolics by Cartridge

Cartridge에 의한 지질과 폐놀물질 혼합산화물의 분리

  • 김정숙 (계명전문대학 식품영양과)
  • Published : 1995.04.01


The oxidized mixture of lipid(methyl linoleate) and phenolics(phloroglucinol) at 37$^{\circ}C$, 82$m\ell$ O/min., for 9 days were separated each by C cartridge and silica cartridge making use of different eluting solvent. And the oxidation products were analyzed by HPLC. In conclusion, the oxidized mixture were separated into methyl linoleate and phloroglucinol by cartridge on HPLC. and also in this experiment, separated methyl linoleate and phloroglucinol could be analyzed in the common eluant, water and acetonitrile on HPLC. SEP-PAK cartridges were used almost sample purification until now, but under the various eluting solvents and conditions, cartridge will be expected to mini columns which can separate different polarity materials.


  1. J. Am. Oil Chem. Soc. v.39 The inital stage of autoxidation Privett,O.S.;Blank,M.L.
  2. J. Am Oil Chem. Soc. v.63 Study on the oxidative rate and prooxidant activity fo free fatty acids Kazuo,M.;Toru,T.
  3. j. Food Sci. v.42 Ferulic acid and other phenolics in oat seed Avena,S.L.;Hinoat,L.V.
  4. J.Food Sci. v.43 Phenolic antioxidants of dried soybeans Patricia,A.;Pratt,D.E.
  5. J.Food Sci. v.51 Role of α-tocopherol, ascorbic acid, citric acid and EDTA as antioxidants in model systems Mahoney,J.R.
  6. J. Agric. Food Chem. v.37 Nonenzymic autoxidative phenolic browning reactions in a caffeic acid model system Cilliers,J.J.L.;singleton,V.L.
  7. Korean J. Food Sci. Tech. v.14 Toxicological studies of antioxidant, BHT and BHA Choe,N.Y.;Yang,Y.H.
  8. J. Am. Oil Chem. Soc. v.52 Toxicology and biochemistry of BHA and BHT Branen,A.L.
  9. J. Am. Oil Chem. Soc. v.60 Pheolic acids in rapeseed and mustard Kozolwska,H.;Zadernowski,R.
  10. Agric. Biol. Chem. v.49 Studies on antioxidative substances sesame seed Yasuk.F.;Toshihiko,O.;Mitsuo,N.
  11. Agric. Biol. Chem. v.41 no.12 Geometrical isomers of monohydroperoxides formed by autoxidation of methyl linoleate Juni,T.;Setsuro,M.
  12. Agric. Biol. Chem. v.46 no.3 Formation of dimer during the initial stage of autoxidation in methyl linoleate Kazuo,M.;Kensiro,F.;Takashi,K.
  13. J. Agric. Food chem. v.38 Caffeic acid autoxidation and the effect of thiols Clilliers,J.J.L.;Signleton,V.L.
  14. Agric. Biol. Chem. v.47 no.9 Some analytical observations of autoxidation products of linolecic acid and their thiobarbituric acid reactive substances Kazuki,K.;Genichi,D.;Masato,N.
  15. J. Sci. Food Agric. v.29 The phenolic cinnamates of white grapes and wine Singleton,V.L.;lea,A.G.H.