Mitotic Stimulation and Cancer Cell Agglutination of the Lectin from Lentinus edodes

표고버섯 렉틴의 림프구 자극 분열 및 암 세포 응집 효과

  • Published : 1995.06.01


A lectin from the edible mushroom, Lentinus edodes, was purified through physiological saline extraction, ammonium sulfate fractionation and column chromatographies. On polyacrylamide gel electrophoresis, 0.05M fraction from hydroxyapatite column exhibited adjacent four sharp bands. The partially purified lectin agglutinated the erythrocytes of rabbit, mouse and rat, but not agglutinated human erythrocytes. The lectin's mitogenic effects were tested by its application to human and murine splenic lymphocytes. The results showed that the 0.05M fraction from hydroxyapatite was mitogenic, and the optimal dose of Lentinus edodes lectin was slightly lower than Con A by the culture with murine splenic and human peripheral lymphocytes. Meanwhile, its ability to agglutinate transformed cells was tested by its administration to continuous cell lines L1210 and HeLa cells. The leetin was found to be an agglutinin of tumor cell lines tested by L1210 and HeLa cells.


  1. Arch Pharm. Res. v.2 Studies on the constituents of higher fungi of Korea(ⅩⅩⅢ) Antineoplastic activities of Coriolus versicolor, Pleurotus ostreatus, Lentinus edodes Kim,B.K.;Park,E.K.;Shim,M.J.
  2. Proc. Symp. Recent Adv. Natural Prod. Res. v.141 Studies on antitumor constitients of higher fungi of Korea Kim,B.K.;Sung,E.;Choi,E.C.
  3. Kor. J. Mycol. v.10 Studies on the constituents of the higher fungi of Korea(Ⅱ);The antitumor components and culture of Lentinus edodes Chung,K.S.
  4. Arch. Pham. Res. v.5 Studies on the constituents and culture of Korean basidiomycetes, antitumor polysaccharides from the mycelia of some basidiomycetes Chung,K.S.;Choi.E.C.;Kim,B.K.;Kim,Y.S.;Park,Y.H.
  5. Kor. J. Mycol. v.10 Studies on constituents of higher fungi of Korea(ⅩⅩⅩⅤ), antitumor components extract from the carphores and cultured mycelia of Laccaria laccata Kim,S.H.;Woo,M.S.;Kim,B.K.
  6. Nature v.285 What should be called a lectin? Goldstein,I.J.;Hughes,R.C.;Monsigny,M.;Osawa,T.;Sharon,N.
  7. Nature v.292 Defining a lectin Dixon,H.B.F.
  8. J. Biol. Chem. v.256 Nomenclature Committee of IUB-IUPAC joint commission and biochemical nomenclature What is a lectin?
  9. The lectin: Properties, function and application in biology and medicine Liener,I.E.;Sharon,N.;Goldstein,I.J.
  10. J. Biol. Chem. v.242 Studies on a hemagglutinin from the mushroom Agaricus campestris Sage,H.J.;Vazquez,J.J.
  11. Biol. Chem. Hoppe-Seyler v.366 Purification and characterization of four isolectins of mushroom(Agaricus bisporus) Sueyoshi,S.;Tsuji,T.;Osawa,T.
  12. Biochemistry v.22 Purification and properties of two hemag glutinins of mushroom Laccaria amethystiena Guillot,J.;Genaud,L.;Gueugnot;Damez,M.
  13. J. Natural Sciences v.6 Studies on lectins from mushrooms(Ⅰ): Isolation of lectinlike agglutinins from mushroom Jeune,K.H.;Chung,S.R.
  14. Yakhak Hoeji v.31 Studies on lectins from mushrooms(Ⅱ): Screening of bioactive substance, lectins, from Korean wild mushrooms Jeune,K.H.;Kim,M.K.;Chung,S.R.
  15. Proceedings of Int. Symp. on New Drug Development from Natrual Products;Kor. Soc. Pharmacogn. v.32 Studies on lectins from Korean higher fungi(Ⅲ) Jeune,K.H.;Kim,M.K.;Chung,S.R.
  16. Planta Med. v.56 Studies on lectins from Korean higher fungi(Ⅳ);A mitogenic lectin from the mushroom Lentinus edodes Jeune,K.H.;Moon,I.J.;Kim,M.K.;Chung,S.R.
  17. Cancer Res. v.30 Fractionation and purification of the polysaccharide with marked antitumor activity, especially lentinan from Lentinus edodes Chihara,G.;Hamuro,T.;Maeda,Y.;Fukuoka,F.
  18. Nature v.229 Lentinan, a new immunoaccelerator of cell mediated responses Maeda,Y.;Chihara,G.
  19. Biol. Cellulaire v.36 Membrane lectins Monsigny,M.;Kieda,C.;Roch,A.C.
  20. Methods in Enzymology v.22 Chromatography of proteins on hydroxyapatite Bernardi,G.
  21. Ann. N.Y. Acad. Sci. v.121 Disc elctrophoresis Ⅱ Davis,B.J.
  22. Arch. Pharm. Res. v.3 Isolation, purification and charac terization of phytohemagglutinating proteins from Korean natural products Chung,S.R.;Jeune-Chung,K.H.;Kim,K.A.
  23. The Antigens Lectins: Their chemistry and application to immunology Lis,H.;Sharon,N.;Sela,M.(ed.)
  24. J. Biol. Chem. v.193 Protein measurement with folin phenol reagent Lowry,O.H.;Rosebrough,N.J.;Farr,A.L.;Randall,J.
  25. Proc. Natl. Acad. Sci. USA v.75 Panning for lymphocytes: A method for cell selection Wysocki,L.J.;Sato,V.L.
  26. Cellular Immunology v.3 Preparation of mouse cell suspensions in selected methods Mishell,B.B.;Shiigi,S.M.;Henry,C.;Chan,E.L.;North,J.;Gallily,R.;Slomich,M.;Miller,K.;Marbrook,J.;Parks,D.;Good,A.H.;Mishell(ed.);Shiigi(ed.)
  27. Lymphokines and Interferons v.345 Assay for human B cell growth and differentiation factors Callard,R.E.;Shields,J.S.;Smith,S.H.;Clemens(ed.);Morris(ed.);Gearing(ed.)
  28. J. Immunol. Methods v.5 Separation of mouse spleen cells by passage through columns of Sephadex G-10 Ly,I.A.;Mishell,R.I.
  29. Manual of Laboratory Immunology v.1 Peacock,J.E.;Tomar,R.H.;Peacock(ed.);Tomar(ed.)
  30. Ph. D. Thesis, Yeungnam University v.1 Studies on lectins from Korean higher fungi Isolation, purification and biochemical properties of lectins from Lactarius piperatus Kim,M.K.
  31. Proc. Natl. Acad. Sci. USA v.50 Reaction of normal and tumor cell surfaces to enzyme, Ⅰ. Wheatgerm lipase and associated mucopolysaccharide Aub,T.C.;Tieslau,C.;Lankester,A.
  32. Molecular action of toxins and viruses v.51 Toxic lectins and related proteins Olsnes,S.;Pihl,A.;Cohen(ed.);Von Heyningen(ed.)
  33. Biochem. Soc. Kor. v.371 Lectins. Review of Biochem. Chung,S.R.;Jeune,K.H.