Construction and Characterization of Escherichia coli-Corynebacterium nephridii Hybrid Thioredoxins

  • Sa, Jae-Hoon (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
  • Kim, Kyung-Hoon (Department of Biology, College of Natural Sciences, Kangwon National University) ;
  • Lim, Chang-Jin (Department of Biochemistry, College of Natural Sciences, Kangwon National University)
  • Received : 0
  • Accepted : 0
  • Published : 0

Abstract

Thioredoxin is a small redox protein with an active-site disulfide/dithiol, and is ubiquitous in bacteria, plants, and animals. To investigate the structure-function relationship of thioredoxin, the genes encoding Escherichia coli thioredoxin and Corynebacterium nephridii thioredoxin C3 were fused via a common restriction site in the nucleotide sequence coding for the active site of the proteins to generate two chimeric thioredoxins, designated E-C3(N to C-terminal) and C3-E. The hybrid thioredoxin genes were put under the T7 promoter and their productions were confirmed. The two hybrid thioredoxins complemented phenotypes of a thioredoxin-deficient E. coli strain. A strain containing the C3-E hybrid thioredoxin supported growth of the T7 phage, whereas a strain expressing the E-C3 hybrid thioredoxin did not. However, both hybrids supported growth of M13 phages. The two hybrid thioredoxins were also characterized in other aspects. Differences in activity between the hybrid thioredoxins were attributed to altered interactions of the N- and C-terminal domains of the molecule, which produced changes in the three-dimensional structure of the active site region.

Keywords

Thioredoxin;Escherichia coli;Corynebacterium nephridii