Adaptive Response Induced by Low Dose Ionizing Radiation in Human Lymphocytes

인체 말초 혈액 림프구에서 저선량 방사선 조사에 의해 유도되는 적응 반응

  • Kim, Jeong-Hee (Korea Atomic Energy Research Institute Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • Lee, Kyung-Jong (Korea Atomic Energy Research Institute Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • Cho, Chul-Koo (Korea Atomic Energy Research Institute Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • Yoo, Seong-Yul (Korea Atomic Energy Research Institute Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • Kim, Tae-Hwan (Korea Atomic Energy Research Institute Laboratory of Radiation Effect, Korea Cancer Center Hospital) ;
  • Kim, Sung-Ho (Korea Atomic Energy Research Institute Laboratory of Radiation Effect, Korea Cancer Center Hospital)
  • 김정희 (한국원자력연구소 부설 원자력병원 방사선영향연구실) ;
  • 이경종 (한국원자력연구소 부설 원자력병원 방사선영향연구실) ;
  • 조철구 (한국원자력연구소 부설 원자력병원 방사선영향연구실) ;
  • 류성렬 (한국원자력연구소 부설 원자력병원 방사선영향연구실) ;
  • 김태환 (한국원자력연구소 부설 원자력병원 방사선영향연구실) ;
  • 김성호 (한국원자력연구소 부설 원자력병원 방사선영향연구실)
  • Published : 1995.06.30

Abstract

Adaptive response induced by low dose ${\gamma}-ray$ irradiation in human peripheral lymphocytes was examimed. Human lymphocytes were exposured to low dose of ${\gamma}-ray$ (priming dose, 0.01Gy) followed by high dose (challenging dose, 1.5Gy) after various time intervals (4, 7, 20 hours). Frequencies of micronuclei were enumerated in both primed and unprimed groups. Maximum reduction in frequency of micronuclei was observed when challenging dose irradiation was followed by priming dose after 4hr incubation period. When challenging doses were irradiated 7 or 20hr after priming dose, frequencies of micronuclei were reduced slighty. However, these reduction were not statistically significant. In this study, human peripheral lymphocytes were irradiated at Go phase and they showed adaptive response induced by low dose radiation. Since micronucleus assay is relatively simpler and faster than other methods, it may be a good tool for evaluating radiation-induced adaptive responses.