Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

The effects of exogenous insulin-like growth factor-I on broiler chicken growth

외인성 insulin-like growth factor-I이 육계의 성장에 미치는 영향

  • Lee, Ho-il (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Moon-joon (Iri Agricultural and Technical College) ;
  • Lee, Dae-yeoul (Medical school, Chonbuk National University) ;
  • Kim, Young-ann (Faculty of pharmacy, Woosuk University) ;
  • Kang, Chang-won (College of Veterinary Medicine, Chonbuk National University) ;
  • Chon, Seung-ki (College of Veterinary Medicine, Chonbuk National University)
  • 이호일 (전북대학교 수의과대학 수의 생리학교실) ;
  • 이문준 (이리 농공 전문 대학) ;
  • 이대열 (전북대학교 의과대학 소아과학 교실) ;
  • 김영안 (우석대학교 약학부) ;
  • 강창원 (전북대학교 수의과대학 수의 생리학교실) ;
  • 전승기 (전북대학교 수의과대학 수의 생리학교실)
  • Received : 1994.05.13
  • Published : 1994.07.30

Abstract

Insulin-like growth factor-I(IGF-I) plays an important role in the regulation of mammalian and poultry growth. IGF-I has many actions in different tissues, which include metabolic, mitogenic, and differentiative actions. IGF-I induces insulin-like effects - such as increased cell glucose uptake and glycogen sysnthesis, however several physiological actions of IGF-I may not have been identified yet. In order to investigate the effect on growth in broiler chicken treated with exogenous insulin-like growth factor-I, 30 chickens were injected $50{\mu}g$ reconbinant human IGF- I (rhIGF- I ) per kg body weight as experimental group and 30 ckickens saline subcutanously as control, 3 times according to ages from 2 to 35 days. We established radioimmunoassay method by which we can measure chicken IGF- I (cIGF- I ) as in rhIGF- I assay. The results obtained were as follows; 1) The dilution curve showed in parallelism between rhIGF- I and cIGF- I in the Sep-pak $C_{18}$ cartridge plasma extracts. 2) The body weight of broiler chicken were significantly increased at 31 days($1,176.50{\pm}99.79g$) and 35 days($1,252.84{\pm}125.21g$) of age in treatment groups, compared with control group($1,011.88{\pm}40.22g,\;1,111.32{\pm}153.67g$). The liver and kidney weights on 35 days$(35.24{\pm}5.18g,\;11.05{\pm}1.47g)$ were significantly higher in rhIGF- I treated group than control group($30.95{\pm}4.04g,\;10.01{\pm}1.60g$) 3) The plasma concentration of IGF- l and total protein in rhIGF- I treated group were $58.17{\pm}1.69ng/ml$, $3.75{\pm}0.62g/dl$ respectively compared with control group $45.70{\pm}1.64ng/ml$, $2.32{\pm}0.53g/dl$. The results suggest that exogenous rhIGF- I increased total body weight, liver and kidney weights in broiler chicken, and it may increase IGF- I and total protein concentration in serum.

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Supported by : 학술 진흥 재단