Development of Soil Dilution Planting Method for Ecological Studies of Pythium Populations

Pythium Population 의 생태적 특성 고찰을 위한 Soil Dilution Planting Method의 개발

  • Lee, Youn-Su (Institute of Agricultural Science, College of Agriculture, Kangwon National University)
  • 이윤수 (강원대 농과대학 농업과학연구소)
  • Published : 1994.04.30


The number of colonies often decreased more than would be expected in a dilution from 1:50 to 1:100; however, a lack of agreement between the dilution series and the number of colonies obtained also occurred at higher dilutions. In the experiments with each soil subsample, there was sometimes poor agreement between the number of colonies obtained at the same dilution from the A and B subsamples. However, repeated 1:50 dilutions of soil suspensions of subsamples A and B yielded similar numbers of colonies. In the second experiment series conducted with a second composite soil sample, the number of colonies obtained from each soil subsample decreased following air drying. The results suggest that it was difficult to obtain a uniform distribution of Pythium propagules in the two sugarcane field soils tested. The high number of propagules detected at the 1:50 dilution could have been due to hyphal fragments or connected hyphal swellings that separated during the final mixing or during plating.