Isolation and Characterization of 2,4,5-Trichlorophenoxyacetic Acid Degrading Bacteria

2,4,5-Trichlorophenoxyacetic Acid 분해균주의 분리 및 특성

  • Park, Yeong-Soon (Dept. of Microbiology, college of Natural Science, Pusan National University) ;
  • Lee, Geon (Dept. of Microbiology, college of Natural Science, Pusan National University) ;
  • Lee, Sang-Joon (Dept. of Microbiology, college of Natural Science, Pusan National University) ;
  • Lee, Jong-Kun (Dept. of Microbiology, college of Natural Science, Pusan National University)
  • 박영순 (부산대학교 미생물학과) ;
  • 이건 (부산대학교 미생물학과) ;
  • 이상준 (부산대학교 미생물학과) ;
  • 이종근 (부산대학교 미생물학과)
  • Published : 1994.09.01


Microorganisms capable of utilizing 2,4,5-trichlorophenoxyacetic acid(2,4,5-T) as sole carbon source were isolated from soil by enrichment culture. Among these strains, EL-O7IP had the highest biodegradability of 2,4,5-7, and according to its morphological and physiological characteristics, it was identified as Pseudomonas sp. This strain was resistant to rifampicin, streptomycin, ampicillin, kanamycin and such metal ions as $Zn^{2+}$, $Cu^{2+}$ Various compounds of chlorinated phenol and substrate analogs were more easily utilized than 2,4,5-7, but biodegradation rate for each compound was different. The strain easily utilized the compounds of chlorinated substituents on phenol in the order of ortho-, para-, and meta- position. The biodegradability of this strain was very stable. Key words : 2,4,5- trichlorophenoxyacetic acid, Pseudomonas sp .


2,4,5-trichlorophenoxyacetic acid;Pseudomonas sp.


  1. Appl. Environ. Microbiol. v.46 Metabolism of halophenols by 2,4,5-T degrading Pseudomonas cepacia Karns,J.S.;J.J.Kilbane;S.Duttagupta;A.M.Chakrabarty
  2. Microbial degradation of xenobiotics and recalcitrant compounds Manupulation of degradative genes of soil bacteria Franklin,F.C.H.;M.Bagdasrain;K.N.Timmis;T.Leisinger(ed.);R.Hutter(ed.);A.M.Cook(ed.);J.Nuesch(ed.)
  3. J. Agric. Food. Chem. v.28 Microbial metabolism of 2,4,5-T in soil, soil suspensions and axenic culture Rosenberg,A.;M.Alexander
  4. J. Agric. Food Chem. v.32 Dechlorination of 2,4,5-T by an anaerobic microorganism Suflita,J.M.;J.Stout;J.M.Tiedje
  5. Science v.212 Aerial spraying of 2,4,5-T and human birth malformation : on epidemiological investigation Hanify,J.A.;P.Metcalt;C.L.Nobbs;K.J.Worsley
  6. Manual of Methods for General Bacteriology Gerhardt,P.;R.G.E.Murray;R.N.Costilow;E.W.Nester;W.A.Wood;N.R.Krieg;G.B.Phillips
  7. Bergey's Manual of Determinative Bacteriology(8th ed.) The William and Wilkins Co.
  8. Appl. Environ. Microbiol. v.49 Degradation of herbicide mecoprop (2-(2-methyl 4-chlorophenoxy) propionic acid) by a synergistic microbial community Hilary,M.C.;P.G.Michael;J.H.Slater
  9. J. Agric. Food Chem. v.28 2,4,5-T Decomposition on tropical soil and its cometabolism by bacteria in vitro Rosenberg,A.;M.Alexander
  10. Bergey's Manual of Determinative Bacteriology v.1 The William and Wilkins Co.
  11. Laboratory Microbiology(3rd ed.) Bradshow,L.J.
  12. J. Bacteriol. v.135 Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5 Hansen,J.B.;R.H.Olsen
  13. Chemistry of the environment Pesticides, polych-lorinated-biphenyls, and other chloroorganic compounds Bailey,R.A.;H.M.Clarke;J.P.Ferris;S.Krause;R.L.Strong
  14. Progress in pesticide biochemistry and toxicology v.4 Biotransformation of pesticides and other xenobiotics in plants and soils-recent developments Edwards,V.T.;A.C.McMinn;D.H.Hutson(ed.);T.R.Roberts(ed.)
  15. Molecular biology, pathogenicity, and ecology of bacterial plasmids Plasmid in the biodegradation of chlorinated aromatic compounds Chatterjee,D.K.;S.T.Kellogg;D.R.Watkins;A.M.Chakrabarty;S.B.Levy(ed.);R.C.Clowes(ed.);E.L.Koening(ed.)
  16. The biochemical mode of action of pesticides(2nd ed.) Compunds interfering with cell growth, division, and development Corbelt,J.R.;K.Wright;A.C.Baillie
  17. Appl. Environ. Microbiol. v.44 Biodegradation of 2,4,5-T in soil by a pure culture of Pseudomonas cepacia Chatterjee,D.K.;J.J.Kilbane;A.M.Chakrabarty
  18. J. Agric. Food Chem. v.29 Fate of uniformly carbon-14-ring labelled 2, 4, 5-T and 2, 4-D McCall,P.J.;S.A.Vrona;S.S.Kelley
  19. Mutat. Res. v.65 The Genotoxic Effects of 2,4,5-T Grant,W.F.
  20. Appl. Environ. Microbiol. v.45 Detoxification 2,4,5-T from contaminated soil by Pseudomonas cepacia Kilbane,J.J.;D.K.Chatterjee;A.M.Chakrabarty
  21. Science v.211 Biodegradation of chemicals of environmental concern Alexander,M.
  22. Science v.288 Microbial degradation of halogenated compounds Chakrabarty,A.M.;D.Ghosal;I.S.You;D.K.Chatterjee
  23. Kor. Jour. Microbial. v.25 Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbon Kim,J.W.;C.K.Kim;T.H.Yeom;J.G.Lee
  24. Biochemical test for identification of medical bacteria MacFaddin,J.F.
  25. Appl. Environ. Microbiol. v.46 Regulation of 2,4,5-T and chlorophenol metabolism in Pseudomonas cepacia AC 1100 Karns,J.S.;S.Duttagupta;A.M.Chakrabarty
  26. Appl. Environ. Microbiol. v.41 Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plate Kiyohara,H.;K.Nagao;K.Yana
  27. 최신 신농약 장판섭
  28. Appl. Environ. Microbiol. v.44 Biodegradation of 2,4,5-T by a pure culture of Pseudomonas cepacia Kilbane,J.J.;D.K.Chatterjee;J.S.Karns;S.T.Kellogg;A.M.Chakrabarty
  29. Science v.214 Plasmid-assisted molecular breeding: new technique for enhanced biodegradation of persistent toxic chemicals Kellogg,S.T.;R.K.Chatterjee;A.M.Chakrabarty
  30. Bull. Enviro. Contam. Toxicol. v.5 Microbial cometabolism of 2,4,5-T Horvath,R.S.