The Effect of Growth Regulators and Light Quality on the Changes in Protein Pattern of Callus from Intergeneric Protoplast Fusion between Nicotiana tabacum and Solanum nigrum

Nicotiana tabacum과 Solanum nigrum의 속간 원형질체 융합에서 유도된 캘러스의 단백질 양태변화에 미치는 생장조절제 및 광선의 효과

  • 김영상 (부산대학교 자연과학대학 생물학과) ;
  • 이동희 (부산대학교 자연과학대학 생물학과)
  • Published : 1994.06.01

Abstract

The effect of growth regulators (NAA, BA and $ extrm{GA}_3$) and light (blue, red and far-red) on the changes in total protein and thylakoid membrane protein pattern of callus from intergeneric protoplast fusion between Nicotiana tabacum and Solanum nigrw were investigated. When the callus were irradiated with different wavelengths of light, blue and red light accelerated the synthesis of total proteins and thylakoid membrane proteins. Particularly, red light led to an increase in the protein synthesis compared to blue light. When the callus were subjected to various combinations of growth regulators, NAA+$ extrm{GA}_3$ and NAA+BA treatments induced remarkable increase of total proteins and thylakoid membrane proteins accumulation, particularly in the combination of NAA+$ extrm{GA}_3$. NAA.$ extrm{GA}_3$ treatment with irradiation of red ligh showed highest value in the accumulation of total proteins and thylakoid membrane proteins. We conclude that simultaneous application of red light and NAA+$ extrm{GA}_3$ treatment may induce synergistic effect in the synthesis of total proteins and thylakoid membrane Proteins.

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