Studies on the Selections, the Cross-Resistance and the Eserase Activity in the Strain of German Cockroack, Blattella germanica L.Selected with dichlorvos

바퀴에 대한 Dichlorvos(DDVP)의 누대도태, 교차저항성 및 효소활성에 관한 연구

  • 이형래 (충북대학교 농과대학 농생물학과) ;
  • 김정화 (충북대학교 농과대학 농생물학과) ;
  • 방종렬 (충북대학교 농과대학 농생물학과) ;
  • 최관선 (충북대학교 농과대학 농생물학과)
  • Published : 1994.06.01

Abstract

The german cockroach, Blattello germanica L, populations were successively selected with dlchlowos for 11 generations. The resulting selected shin was investigated the resistance development. the cross resistance and the esterase actim@. In the dichlowos-selected(Rd) strain, the values of LCs increased 858 times more compared to the susceptible (S) strain. In the dich!o~vos-selected (Rd) strains, the cross-res~stance to chlorpyrifos, propoxur, fenvalerate and pemethnn showed 3.35. 4.09. 283 and 2.00 times. Esterase.activity of the Rd strain showed 1.33 times higher than that of the S strain in the filter paper test. In comparison of zymogram paitems of the estemse isoryme by thin agarose gel electrophoresis against the german cockroach, the S strain was separated by 4 bands uf esterase 3, 5, 6 and 8 bands and the Rd strain was separated by 6 bands of esterase 1, 2, 4, 5, 7 and 8 bands, and the resistant mechanisms of the Rd strain were considered as the 4 bands of esterase-1.2.4 and 7 bands except the common 2 bands of esterase.6 and 8 bands.

Keywords

dichlorvos;German cockroach;insecticide resistance;dichlorvos