Isolation and Characterization of a Phenol-Degrading Strain Acinetobacter sp.GEM2

Phenol을 분해하는 Acinetobacter sp. GEM2의 분리 및 특성

  • Lee, Chang-Ho ;
  • Oh, Hee-Mock ;
  • Kwon, Tae-Jong ;
  • Kwon, Gi-Seok ;
  • Lee, Sung-Gie ;
  • Suh, Hyun-Hyo ;
  • Yoon, Byung-Dae
  • 이창호 (한국과학기술연구원 유전공학연구소) ;
  • 오희목 (한국과학기술연구원 유전공학연구소) ;
  • 권태종 (건국대학교 미생물공학과) ;
  • 권기석 (한국과학기술연구원 유전공학연구소) ;
  • 이성기 (한국과학기술연구원 유전공학연구소) ;
  • 서현효 (한국과학기술연구원 유전공학연구소) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소)
  • Published : 1994.12.01


A bacterial strain which formed a distinct colony on agar plate containing phenol as a vapor phase and grew well in a liquid minimal medium was isolated and identified as Acinetobac- ter sp. GEM2. The optimal temperature and initial pH for the growth of Acinetobacter sp. GEM2 were 30$\circ$C and 7.0, respectively. Cell growth was inhibited by phenol at the concentration over 1500 ppm. Cell growth dramatically increased from 10 hours after cultivation and almost showed a stationary phase within 24 hours at which 95% of phenol was concomitantly degraded. Acinetobac- ter sp. GEM2 was capable of growing on aromatic compounds, such as benzoic acid, phenol, m- cresol, o-cresol, P-cresol, catechol, gentisic acid, and toluene, but did not grow on benzene, salicylic acid, p-toluic acid, and p-xylene. By the analysis of catechol dioxygenase, it seemed that catechol was degraded through both meta- and ortho-cleavage pathway. The growth-limiting log P value of Acinetobacter sp. GEM2 on organic solvents was 2.0.


Acinetobacter sp. GEM2;phenol;aromatic compuounds


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