Isolation and Characterization of a Phenol-Degrading Strain Acinetobacter sp.GEM2

Phenol을 분해하는 Acinetobacter sp. GEM2의 분리 및 특성

  • Lee, Chang-Ho ;
  • Oh, Hee-Mock ;
  • Kwon, Tae-Jong ;
  • Kwon, Gi-Seok ;
  • Lee, Sung-Gie ;
  • Suh, Hyun-Hyo ;
  • Yoon, Byung-Dae
  • 이창호 (한국과학기술연구원 유전공학연구소) ;
  • 오희목 (한국과학기술연구원 유전공학연구소) ;
  • 권태종 (건국대학교 미생물공학과) ;
  • 권기석 (한국과학기술연구원 유전공학연구소) ;
  • 이성기 (한국과학기술연구원 유전공학연구소) ;
  • 서현효 (한국과학기술연구원 유전공학연구소) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소)
  • Published : 1994.12.01

Abstract

A bacterial strain which formed a distinct colony on agar plate containing phenol as a vapor phase and grew well in a liquid minimal medium was isolated and identified as Acinetobac- ter sp. GEM2. The optimal temperature and initial pH for the growth of Acinetobacter sp. GEM2 were 30$\circ$C and 7.0, respectively. Cell growth was inhibited by phenol at the concentration over 1500 ppm. Cell growth dramatically increased from 10 hours after cultivation and almost showed a stationary phase within 24 hours at which 95% of phenol was concomitantly degraded. Acinetobac- ter sp. GEM2 was capable of growing on aromatic compounds, such as benzoic acid, phenol, m- cresol, o-cresol, P-cresol, catechol, gentisic acid, and toluene, but did not grow on benzene, salicylic acid, p-toluic acid, and p-xylene. By the analysis of catechol dioxygenase, it seemed that catechol was degraded through both meta- and ortho-cleavage pathway. The growth-limiting log P value of Acinetobacter sp. GEM2 on organic solvents was 2.0.

Keywords

Acinetobacter sp. GEM2;phenol;aromatic compuounds

References

  1. Biotechnol. Lett. v.9 Selection and adaptation of a phenol-degrading strain of Pseudomonas Masque,C.;M.Nolla;A.Bordons
  2. Appl. Microbiol. Biotechnol. v.37 Degradation of phenol and phenolic compounds by Pseudomonas putida EKII Hinteregger,C.;R.Leitner;M.Loidl;A.Fersch;F.Streichsbier
  3. J. Bacteriol. v.107 Dissimilation of aromatic compounds by Alcaligenes eutrophus Johnson,B.F.;R.Y.Stanier
  4. J. Bacteriol v.91 Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida.Ⅰ.Synthesis of the enzymes by the wild type Hegeman,G.D.
  5. Biotechnol. Lett. v.11 Kinetics and fluorometric behaviour of a phenol fermentation Li,J.K.;A.E.Humphrey
  6. Methods in Enzymology 17A Metapyrocatechase(Pseudomonas) Nozaki,M.
  7. J. Gen. Appl. Microbiol. v.29 The coenzyme Q system in strains of some yeasts and yeast-like fungi Yamada,Y.;T.Ohishi;K.Kondo
  8. Appl. Environ. Microbiol. v.58 Physiological properties of a Pseudomonas strain which grows with ρ-xylene in a two-phase(organic-aqueous) medium Cruden,D.L.;J.H.Wolfram;R.D.Rogers;D.T.Gibson
  9. 산업미생물학회지 v.17 Pseudomonas 속 세균에 의한 방향족화합물 생분해 정윤창;김경남;최용진;양한철;송준상;서윤수
  10. J. Fermen. Bioeng. v.71 Estimation of solvent-tolerance of bacteria by the solvent parameter Log P. Inoue,A.;K.Horikoshi
  11. Chemical Method in Bacteria Systematics Goodfellow,M.;D.E.Minnikin
  12. 산업미생물학회지 v.16 Naphthalene을 분해하는 Pseudomonas putida N3의 분리 및 특성 고영희;하일호;배경숙
  13. Appl. Microbiol. Biotechnol. v.26 Degradation of phenol by a mixed culture of Pseudomonas putida and Cryptococuus elinovii adsorbed on activated carbon Morsen,A.;H.J.Rehm
  14. J. Bacteriol. v.85 Classification of microorganisms by analysis of chemical composition:Ⅰ. Flaability of utilizing gas chromatography Abel,K.;H.de Schmertzing;J.I.Peterson
  15. Bergey's Manual of Systematic Bacteriology Krieh,N.R.;K.J.Steel
  16. Agric. Biol. Chem. v.55 Preparation of organic solvent-tolerant mutants from Escherichia coli K-12 Rikizo,A.;K.Aibe;A.Inoue;K.Horikoshi
  17. FEMS Microbiol. Lett. v.25 Determination of DNA base composition by reversed-phase HPLC Tamaoka,J.;K.Komagata
  18. Biotech. Bioeng. v.17 Substrate inhibition kinetics:phenol degradation by Pseudomonas putida Gordon,A.H.;W.R.Compbell
  19. Appl. Microbiol. Biotechnol. v.30 Semicontinuous and continuous degradation of phenol by Pseudomonas putida P8 adsorbed on activated carbon Ehrhardt,H.M.;H.J.Rehm
  20. J. Bacteriol. v.113 Metabolism of phenol and cresols by mutant of Pseudomonas putida Bayly,R.C.;G.J.Wigmore
  21. Manual for the Identification of Medical Bacteria(2nd ed.) Cowan,N.R.;K.J.Steel
  22. Hewlett-Packard Application:Note Bacteria identification by gas chromatography of whole cell fatty acid Miller,L.T.;T.Berger
  23. 산업미생물학회지 v.19 방향족화합물의 함유된 폐수의 생물학적 처리 박춘호;김용기;오평수