The Biodegradation of Mixtures of Benzene,Phenol,and Toluene by Mixed and Monoculture of Bacteria

단일배양 및 혼합배양에 의한 Benzene, Phenol 및 Toluene 혼합물의 생분해

  • Lee, Chang-Ho ;
  • Oh, Hee-Mock ;
  • Kwon, Tae-Jong ;
  • Kwon, Gi-Seok ;
  • Kim, Seong-Bin ;
  • Kho, Yung-Hee ;
  • Yoon, Byung-Dae
  • 이창호 (한국과학기술연구원 유전공학연구소) ;
  • 오희목 (한국과학기술연구원 유전공학연구소) ;
  • 권태종 (건국대학교 미생물공학과) ;
  • 권기석 (한국과학기술연구원 유전공학연구소) ;
  • 김성빈 (한국과학기술연구원 유전공학연구소) ;
  • 고영희 (한국과학기술연구원 유전공학연구소) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소)
  • Published : 1994.12.01

Abstract

The biodegradation of aromatic compounds by mixed and monoculture was investigated in an artificial wastewater containing 500 mg/l of benzene(B), phenol(P), and toluene(T) in various combinations. None of three strains utilized P-xylene(X) as a carbon source, but they grew well on p-xylene in mixtures with benzene and toluene. In the mixed culture on mixed substrate, the length of lag phase was different depending on the nature of mixture. Cell growths of Flavobac- terium sp. BEN2 and Acinetobacter sp. GEM63 were inhibited in the presence of a 500 mg/l of phenol. When the mixed culture of three strains was cultured in a bench-scale reactor containing artificial wastewater, each of benzene, phenol, and toluene was not detected at 30 hrs, 50 hrs, and 12 hrs after incubation in the treatment. The removal rates of COD$_{t}$(total COD) and COD$_{s}$,(soluble COD) of upper phase after centrifugation during early 50 hrs were ca. 80% and ca. 93.8%, respectively.

Keywords

Biodergradation;aromatic compounds;xenobiotics

References

  1. Environ. Prog. v.2 Industrial wastewater treatment with a new biological fixed-film system Stensel,H.D.;S.Reiber
  2. Biotechnol. Bioeng. v.33 Dual substrate removal by an axenic bacterial culture Machado,R.J.;C.P.L.Grady
  3. Environ. Toxicol. Chem. v.3 Inhibitory interactions of aromatic organics during microbial degradation Meyer,J.S.;M.D.Marcus;H.L. Bergman
  4. Phil. Trans. R. Soc. Lond. Ser. B. v.297 Co-metabolism Dalton,H.;D.I.Stirling
  5. Environ, Prog v.10 Bioreactor design effects on biodegradation capabilities of VOCs in wastewater Hill,G.A.;M.E.Tomusiak;B.Quail;K.M.Van Cleave
  6. Environ. Sci. Technol. v.16 Microbial removal of hazardous organic compounds Kobayashi,H.;B.E.Rittman
  7. Bacteriol. Rev. v.36 Microbial co-metabolism and the degradation of organic compounds in nature Horvath,R.S.
  8. Appl. Environ. Microbiol v.55 Substrate interactions during aerobic biodegradation of benzene Arvin,E.;B.K.Jensen;A.T.Gunderson
  9. Biotechnol. Bioeng. v.37 The biodegradation of mixtures of organic solvents by mixed and monocultures of bacteria Bitzi,U.;T.Egli;G.Hammer
  10. Soil Biol. Biochem. v.21 Kinetics of biodegradation of mixtures of substrates in soil Scow,K.M.;S.K.Schmidt;M.Alexander
  11. Appl. Microbiol. Biotechnol. v.39 Degradation of polycyclic aromatic hydrocarbons by an immobilized mixed bacterial culture Wiesel,I.;S.M.Wubker;H.J.Rehm
  12. Water Environ. Res. v.65 Fate of volatile organic compounds in municipal activated sludge plants Parker,W.J.;D.J.Thompson;J.P.Bell;H.Melcer
  13. Res. J. Water Pollut. Control. Fed. v.62 Kinetics of chlorinated hydrocarbon degradation by suspended cultures of membrane-oxidizing bacteria Strand,S.E.;M.D.Bjelland;H.D.Stensel
  14. 환경오염공정시험법 동화기술 편집위원회
  15. Biotechnol. Prog. v.9 Simultaneous biodegradation of toluene and ρ-xylene in a novel bioreactor;Experimental results and mathmatical analysis Lee,J.Y.;Y.B.Choi;H.S.Kim
  16. Appl. Environ. Microbiol. v.49 Effects of dissolved organic carbon and second substrates on the biodegradation of organic compounds at low concentrations Schmidt,S.K.;M.Alexander
  17. 산업미생물학회지 v.16 Naphtalene을 분해하는 Pseudomonas putida N3의 분리 및 특성 고영희;하일호;배경숙
  18. Biotechnol. Bioeng. v.41 Kinetics of competitive inhibition and cometabolism in the biodegradation pf benzene, toluene, and p-xylene by two Pseudomonas isolates Chang,M.K.;T.C.Voice;C.S.Criddle