The Biodegradation of Mixtures of Benzene,Phenol,and Toluene by Mixed and Monoculture of Bacteria

단일배양 및 혼합배양에 의한 Benzene, Phenol 및 Toluene 혼합물의 생분해

  • Lee, Chang-Ho ;
  • Oh, Hee-Mock ;
  • Kwon, Tae-Jong ;
  • Kwon, Gi-Seok ;
  • Kim, Seong-Bin ;
  • Kho, Yung-Hee ;
  • Yoon, Byung-Dae
  • 이창호 (한국과학기술연구원 유전공학연구소) ;
  • 오희목 (한국과학기술연구원 유전공학연구소) ;
  • 권태종 (건국대학교 미생물공학과) ;
  • 권기석 (한국과학기술연구원 유전공학연구소) ;
  • 김성빈 (한국과학기술연구원 유전공학연구소) ;
  • 고영희 (한국과학기술연구원 유전공학연구소) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소)
  • Published : 1994.12.01


The biodegradation of aromatic compounds by mixed and monoculture was investigated in an artificial wastewater containing 500 mg/l of benzene(B), phenol(P), and toluene(T) in various combinations. None of three strains utilized P-xylene(X) as a carbon source, but they grew well on p-xylene in mixtures with benzene and toluene. In the mixed culture on mixed substrate, the length of lag phase was different depending on the nature of mixture. Cell growths of Flavobac- terium sp. BEN2 and Acinetobacter sp. GEM63 were inhibited in the presence of a 500 mg/l of phenol. When the mixed culture of three strains was cultured in a bench-scale reactor containing artificial wastewater, each of benzene, phenol, and toluene was not detected at 30 hrs, 50 hrs, and 12 hrs after incubation in the treatment. The removal rates of COD$_{t}$(total COD) and COD$_{s}$,(soluble COD) of upper phase after centrifugation during early 50 hrs were ca. 80% and ca. 93.8%, respectively.


Biodergradation;aromatic compounds;xenobiotics


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