Production of Vanillic Acid from Vanillin by Pseudomonas sp. GD-088

Pseudomonas sp. GD-088에 의한 Vanillin으로부터 Vanillic Acid의 생산

  • 송정화 (고려대학교 자연자원대학 농화학과) ;
  • 이일석 (고려대학교 자연자원대학 농화학과) ;
  • 방원기 (고려대학교 자연자원대학 농화학과)
  • Published : 1994.12.01

Abstract

For production of vanillic acid from vanillin, optimum culture conditions for Pseudomo- nas sp. GD-088, having vanillin-oxidizing activity were investigated. The highest vanillin-oxidizing activity was obtained when this strain was cultured at 30$\circ$C for 24 hr in a medium consisting of 3.0 g/l xylose and 0.46 g/l NH$_{4}$CI (pH 7.0). When 18 g/l of whole Pseudomonas sp. GD-088 cells as the enzyme source was used in 50 mM phosphate buffer (pH 7.0) containing 3.0 g/l of vanillin, 2.463 g/l of vanillic acid was produced for 40 minutes. This amount of vanillic acid corresponds to a 90% yield, based on vanillin.

Keywords

Vanillin;vanillic acid;Pseudomanas sp.

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