Production of Vanillic Acid from Vanillin by Pseudomonas sp. GD-088

Pseudomonas sp. GD-088에 의한 Vanillin으로부터 Vanillic Acid의 생산

  • 송정화 (고려대학교 자연자원대학 농화학과) ;
  • 이일석 (고려대학교 자연자원대학 농화학과) ;
  • 방원기 (고려대학교 자연자원대학 농화학과)
  • Published : 1994.12.01


For production of vanillic acid from vanillin, optimum culture conditions for Pseudomo- nas sp. GD-088, having vanillin-oxidizing activity were investigated. The highest vanillin-oxidizing activity was obtained when this strain was cultured at 30$\circ$C for 24 hr in a medium consisting of 3.0 g/l xylose and 0.46 g/l NH$_{4}$CI (pH 7.0). When 18 g/l of whole Pseudomonas sp. GD-088 cells as the enzyme source was used in 50 mM phosphate buffer (pH 7.0) containing 3.0 g/l of vanillin, 2.463 g/l of vanillic acid was produced for 40 minutes. This amount of vanillic acid corresponds to a 90% yield, based on vanillin.


Vanillin;vanillic acid;Pseudomanas sp.


  1. In Wood-Chemistry, Ultrastructure, Reactions Reactions of lignin Dietrich,F.;W.Gerd
  2. 고려대학교 석사학위논문 Pseudomonas sp.에 의한 vanillin으로 부터 vanillic acid의 생산 송정화
  3. Appl. Environ. Microbiol. v.45 Wholecell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus Anthony,ⅢL.P.;D.L.Crawford
  4. Arch. Microbiol. v.131 Production of an aromatic aldehyde oxidase by Streptomyces viridosporus Crawford,D.L.;J.B.Sutherland;A.L.PomettoⅢ;J.M.Miller
  5. Appl. Environ. Microbiol. v.55 Production of vanillic acid from vanillin by resting cells of Serratia marcescens Perestero,F.;M.A.Falcon;G.De la Fuente
  6. Soil. Sci. v.7 The oxidation of vanillin to vanillic acid by certain soil bacteria Lobbins,W.J.;E.C.Lathrop
  7. Arch. Microbiol. v.125 Vanillic acid metabolism by the white-rot fungus Sporotrichum pulverulentum Paul,A.;H.Annele;E.E.Karl
  8. Biochemistry v.9 The degradation of trans-ferulic acid by Pseudomonas acidovorans Anne,T.;J.M.Wood
  9. In Wood-Chemistry, Ultrastructure, Reactions Utilization of technical lignins Dietrich,F.;W.Gerd