Purification and Characterization of Extracellular $\beta$-Xylosidase from Fungi

곰팡이가 생산하는 세포외 $\beta$-Xylosidase의 정제 및 특성

  • 고명선 (부산대학교 자연과학대학 미생물학과) ;
  • 이상준 (부산대학교 자연과학대학 미생물학과) ;
  • 이종근 (부산대학교 자연과학대학 미생물학과)
  • Published : 1994.12.01

Abstract

The $\beta$-xylosidase from Penicillium sp. FX-102 was purified by 40~80% ammonium sulfate saturation, CM-Cellulose column chromatography, Sephadex G-200 gel filtration, and isoelec- tric focusing. The optimum pH and temperature for the activity of the $\beta$-xylosidase was pH 4.5 and 50$\circ$C, respectively. The enzyme was stable at the pH range of 4.5~5.5, and at 55$\circ$C for 10 min. The molecular weight of the enzyme was estimated to be about 300,000 daltons by Sephadex G-200 gel filtration and 310,000 daltons of monomer by SDS polyacrylamide gel electrophoresis. Isoelectric point of the enzyme was determined to be pH 4.4. The enzyme activity was strongly inhibited by Hg$^{2+}$, Ag$^{2+}$, n-bromosuccinimide and p-chloromercuribenzoate. Xylobiose (10 mM) was completely decomposed to xylose after 8 hrs enzyme reaction with 2 units of the $\beta$-xylosidase.

Keywords

DDS;${\beta}$-xylosidase;purification;characterization

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