Purification and Characterization of Extracellular $\beta$-Xylosidase from Fungi

곰팡이가 생산하는 세포외 $\beta$-Xylosidase의 정제 및 특성

  • 고명선 (부산대학교 자연과학대학 미생물학과) ;
  • 이상준 (부산대학교 자연과학대학 미생물학과) ;
  • 이종근 (부산대학교 자연과학대학 미생물학과)
  • Published : 1994.12.01


The $\beta$-xylosidase from Penicillium sp. FX-102 was purified by 40~80% ammonium sulfate saturation, CM-Cellulose column chromatography, Sephadex G-200 gel filtration, and isoelec- tric focusing. The optimum pH and temperature for the activity of the $\beta$-xylosidase was pH 4.5 and 50$\circ$C, respectively. The enzyme was stable at the pH range of 4.5~5.5, and at 55$\circ$C for 10 min. The molecular weight of the enzyme was estimated to be about 300,000 daltons by Sephadex G-200 gel filtration and 310,000 daltons of monomer by SDS polyacrylamide gel electrophoresis. Isoelectric point of the enzyme was determined to be pH 4.4. The enzyme activity was strongly inhibited by Hg$^{2+}$, Ag$^{2+}$, n-bromosuccinimide and p-chloromercuribenzoate. Xylobiose (10 mM) was completely decomposed to xylose after 8 hrs enzyme reaction with 2 units of the $\beta$-xylosidase.




  1. Agric. Biol. Chem. v.51 Four types of β-xylosidase from Penicillium wort-manni IFO 7237 Matsuo,M.;A.Fujie;M.Win;T.Yasui
  2. J. Biol. Chem. v.195 Somogyi,M.J.
  3. Agric. Biol. Chem. v.48 Purification and some properties of β-xylosidase from Trichodermaviride Matsuo,M.;T.Yasui
  4. Kor. J. Appl. Microbiol. Bioeng. v.15 Purification and properties of extracellular β-xylosidase from alkalophilic Bacillus sp. K-17 Sung,N.K.;I.S.Kang;H.K.Chun;T.Akiba;K.Horikoshi
  5. Appl. Environ. Microbiol. v.153 Induction of celluloytic and xylanolytic enzylme system in Streptomyces sp. Mackenzie,C.R.;D.Bilous;H.Schneider;K.G.Johnson
  6. J. Biol. Chem. v.244 The reliability of molecular weights determinations by dodecyl sulfate-polyacrylamide gel eletrophoresis Weber,K.;M.Osborn
  7. Appl. Environ. Microbiol. v.48 Production and properties of extracellular endoxylanase form Neurospora crassa Mishra,C.;S.Keskar;M.Rao
  8. J. Amer. Chem. Soc. v.56 The determination of enzyme dissociation constants Lineweaver,H.;D.Burk
  9. Agric. Biol. Chem. v.52 Transxylosylation products of xylobiose by β-xylosidase from Penicillium wortmanni IFO 7237 Win,M.;Y.Kamiyama;M.Matsuo;T.Yasui
  10. Agric. Biol. Chem. v.48 Purification and some properties of β-xylosidase form Emericella nidulans Matsuo,M.;T.Yasui
  11. Biochen. J. v.91 Proteins by sephadex gel filtration Lowry,O.H.;N.J.Rosebrough;A.L.Farr;R.J.Randal
  12. Bio Chem. Biophys. Acta. v.482 Grover,A.K.;D.D.Macmurchie;R.J.Cushley
  13. Biochem. J. v.96 The gel filtration behavior of proteins related to their molecular weights over a wide range Andrews,P.
  14. Agric. biol. Chem. v.49 Purification and some properties of Chaetomium trilaterale β-xylosidase Uziie,M.;M.Matsuo;T.Yasui
  15. Nature v.227 Dubois,M.;K.A.Hamilton;P.A.Rebers;F.U.K.Laemmi
  16. Eur. J. Biochem. v.102 De Bruyne,C.K.;G.M.Aerts;R.L.De Gussem
  17. Agric. Biol. Chem. v.41 no.48 Purification and some properties of β-xylosidase from Malbranchea pulchella var. sulfurea Matsuo,M.;T.Yasui;T.Kobayashi
  18. Agric. Biol. Chem. v.50 Purification and characterization of extracellular β-glucosidase from Aspergillus fumigatus Kitpreechavanich,V.;M.Hayashi;S.Nagai
  19. 醱酵工學 v.58 セルロス性 資源の再利用 田中三男
  20. Ann. N.Y. Acad. Sci. v.121 Disc electrophoresis.Ⅱ. Method and application to human serum proteins Davis,B.J.
  21. Method in Enzymology 22 Vesterberg,O.
  22. J. Ferment. Technol. v.62 Inducers for xylanase production by Cryptococcus flavus Yasui,T.;B.T.Nguyen;K.Nakanishi
  23. J. Biochem. v.73 Takenishi,S.;Y.Tsujisaka;J.Fukumoto
  24. Agric. biol. Chem. v.49 Possible identity of xylosidase and β-glucosidase of Chaetomium trilaterale Uziie,M.;M.Matsuo;T.Yasui
  25. Bio. Chem. Biophys. Acta v.482 Grover,A.K.;R.J.Cushley
  26. Agric. Biol. Chem. v.41 no.48 Enzamatic properties of β-xylosidase from Malbranchea pulchella var. sulfurea Matsuo,M.;T.Yasui;T.Kobayashi
  27. Kor. J. Appl. Microbiol. Bioeng. v.11 Studies of hemicellulose system in Aspergillus niger(Ⅱ) Lee,H.J.;Y.D.Choi;M.H.Han