Inhibitory Effects of Cysteine on Growth and Protease Production of Preudomonas sp. RP-222 and its Mutant MR-3966

Pseudomonas sp. RP-222와 변이주 MR-3966 의 생육 및 Protease 생산에 Cysteine이 미치는 저해효과

  • Lee, Kwang-Soo ;
  • Kang, Shin-Kwon ;
  • Son, Bong-Soo ;
  • Roh, Jong-Soo ;
  • Kim, Gyeong-Sook ;
  • Chun, Sung-Sik ;
  • Sung, Nack-Kie
  • 이광수 (경상대학교 식품공학과) ;
  • 강신권 (경상대학교 식품공학과) ;
  • 손봉수 (경상대학교 식품공학과) ;
  • 노종수 (부산 동의공업전문대학 환경공학과) ;
  • 김경숙 (일본 국립건강영양연구소) ;
  • 전성식 (경상대학교 식품공학과) ;
  • 성낙계 (경상대학교 식품공학과)
  • Published : 1994.12.01

Abstract

Cysteine showed strong inhibitory effect on growth and protease production of Pseudo- monas sp. RP-222 and its mutant, MR-3966. Mid- to late-log phase cells were most sensitive to the presence of 10 mM cysteine. The inhibition caused by cysteine was almost completely overcome by addition of isoleucine, leucine and valine mixture to the medium, and inclusion of iso#leucine alone could greatly reduce the inhibitory effects of cysteine. Homocysteine and #cysteine, sulfur compounds having similar structure as cysteine, inhibited to varying degrees the growth of both strains. Cysteine and homocysteine were strong inhibitors of threonine deaminase but not transa#- minase B. These results suggest a relationship between the growth-inhibitory effects of cysteine and other sulfur compounds and the inhibition of isoleucine synthesis at the level of threonine deaminase.

Keywords

Protease;Pseudomonas sp. RP-222;Pseudomonas sp. MR-3966;cysteine

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