Cloning of Biosurfactant-Producing Gene from Bacillus subtilis KL-57

Bacillus subtilis KL-57로부터 생산되는 생체계면활성제 합성 유전자 클로닝

  • 강상모 (건국대학교 공과대학 미생물공학과) ;
  • 이병옥 (건국대학교 공과대학 미생물공학과) ;
  • 이철수 (건국대학교 공과대학 미생물공학과)
  • Published : 1994.12.01

Abstract

A bacterium KL-57 which exhibited biosurfactant activity was isolated. This bacterium was identified as Bacillus subtilis. The biosurfactant-producing gene of B. subtilis KL-57 was cloned into R subtilis MI113 by using plasmid pTB523. The plasmid DNA from the clone was found to carry a 18 kb PstI insert. The biosurfactant-producing gene was cleaved into 4 fragments by SmaI, 3 fragments by PvulI or EcoRl, 4 fragments by PvulI and EcoRI double digestion, 5 fragments by AccI, and 2 fragments by KpnI, HindIII or BamHI. By subcloning the 18 kb Pstl insert, a 2.3 kb EcoRl fragment conferred the biosurfactant producing activity on B. subtilis cells. The 2.3 kb had one HindIII cleave site. But Two fragments, which corresponds HindIII/EcoRl termini, exhibited no biosurfactant activity.

Keywords

Biosurfactant;Bacilluus subtilis;surfactin;gene cloning

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