Improvement of Ethanol-Tolerance of Haploid Saccharomyces diastaticus

반수체 Saccharomyces diastaticus의 에탄올내성 증진

  • Song, Sang-Ho (Center For Genetic Engineering Research, The University of Suwon) ;
  • Kim, Keun (Center For Genetic Engineering Research, The University of Suwon) ;
  • Lee, Min-Woong (Department of Agrobiology, Dongguk university)
  • 송상호 (수원대학교 유전공학연구소) ;
  • 김근 (수원대학교 유전공학연구소) ;
  • 이민웅 (동국대학교 농생물학과)
  • Published : 1994.12.01


Several mutation procedures have been compared to obtain an ethanol-tolerant Saccha- romyces diastaticus strain secreting glucoamylase. These procedures include spontaneous mutation, EMS treatment, UV irradiation, and combination of EMS treatment and UV irradiation. All these methods were followed by adaptation of the yeast cells to gradually higher ethanol concentration. Among these procedures, the combined method of EMS treatment and UV irradiation gave the promising result, i.e. the ethanol tolerance of the yeast increased from 11.5%(v/v) to 14.0%(v/v). Respiratory deficient petite mutants of industrial and ethanol-tolerant yeast strains have been isola- ted and hybridized with haploid S. diastaticus strains. The resulting hybrids showed increased ethanol tolerance and starch-fermentability.


Starch-fermenting yeast;ethanol tolerance;mutation;rare-mating


  1. Biomass Conversion Processes for Energy and Fuels Ethanol production by fermentation Brandt,D.;Sofer,S.S.(ed.);O.R.Zaborsky(ed.)
  2. Eur. J. Appl. Microbiol. Biotechnol. v.16 Isolation of ethanol-tolerant mutants of yeast by continuous selection Brown,S.W.;S.G.Oliver
  3. Laboratory Course Manual for Methods in Yeast Genetics Sherman,F.;G.R.Fink;J.B.Hicks
  4. Appl. Microbiol. Biotechnol. v.22 Direct alcoholic fermentation of starch biomass using amylolytic yeast strains in batch and immobilized cell systems Amin,G.;R.De Mot.;K.Van Dijck;H.Verachtert
  5. Fol. Microbiol. v.16 Selection of high ethanol-yielding Saccharomyces.Ⅰ. Ethanol tolerance and the effect of training in Saccharomyces cerevisiae Hansen Ismail,A.A.;A.M.M.Ali
  6. Appl. Environ. Microbiol. v.54 New amylolytic yeast strains for starch and dextrin fermentation Laluce,C.;M.C.Bertolini;J.R.Ernandes;A.V.Martini;A.Martini
  7. Appl. Environ. Microbiol. v.53 Adaptation of yeast cell membranes to ethanol Jimenez,J.;T.Benitez
  8. Adv. Appl. Microbiol. v.26 Ethanol production by fermentation : An alternative liquid fuel Kosaric,N.;D.C.M.Ng;G.S.Stewart
  9. Methods of Enzymatic Analysis v.3 Ethanol determination with alcohol dehydrogenase and NAD Bernet,E.;I.Gutmann;H.U.Bergmeyer(ed.)
  10. Eur. J. Appl. Microbiol. Biotechnol. v.15 The effect of temperature on the ethanol tolerance of the yeast, Saccharomyces uvarum Brown,S.W.;S.G.Oliver
  11. Biotechnol. Lett. v.8 Efficiency of genetically engineered yeast in the production of ethanol from dextrinized cassava starch Russell,I.;C.M.Crumplen;R.M.Jones;G.G.Stewart
  12. Yeast v.2 The utilization of starch by yeasts McCann,K.;J.A.Barnett
  13. Yeast Biotechnology Classical genetic and protoplast fusion techniques in yeast Jones,R.M.;Berry,D.R.(ed.);I.Russell;G.G.Stewart
  14. J. Am. Soc. Brew. Chem. v.43 Valuable techniques in the genetic manipulation of industrial yeast strains Russell,I.;G.G.Stewart
  15. Process Biochem v.16 Alcohol fermentation by yeasts-the effect of environmental and other variables Jones,R.P.;N.Pamment;P.F.Greenfield
  16. Enzyme Microb. Technol. v.9 Ethanol tolerance of yeast D'amore,T.;G.G.Stewart
  17. Appl. Environ. Microbiol. v.48 Development of rapidly fermenting strains of Saccharomyces diastaticus for direct conversion of starch and dextrins to ethanol Laluce,C.;J.R.Mattoon
  18. In Experiments in Molecular Genetics Ultraviolet light mutagenesis Miller,J.H.
  19. Can. J. Genet. Cytol. v.7 Genetical mutants induced by ethylmethansulfonate(EMS) in Saccharomyces Lindergren,G.;Y.L.Hwang;Y.Oshima;C.C.Lindergren
  20. Fol. Microbiol. v.16 Selection of high ethanol-yielding saccharomyces.Ⅱ. Genetics of ethanol tolerance Ismail,A.A.;A.M.M.Ali
  21. Genetics v.62 Genetic control of radiation sensitivity in Saccharomyces cerevisiae Resnick,M.A.
  22. Appl. Environ. Microbiol. v.45 Selection of wine yeasts for growth and fermentation in the presence of ethanol and sucrose Benitez,T.;L.Del Castillo;A.Aguilera;J.Conde;E.Cerdaolmedo
  23. Appl. Microbiol. Biotechnol. v.22 Potentialities and limitations of direct alcoholic fermentations of starch material with amylolytic yeasts Mot,R.;K.Van Dijck;A.Donkers;H.Varachtfert
  24. Appl. Microbiol. Biotechnol. v.35 Breeding of a distiller's yeast by hybridization with a wine yeast Miklos,I.;M.Sipiczki
  25. J. Microbiol. Biotechnol. v.4 Construction of a transformed yeast strain secreting both α-amylase and glucoamylase for direct starch-fermentaion Kim,K.;J.W.Lee