Improvement of Ethanol-Tolerance of Haploid Saccharomyces diastaticus

반수체 Saccharomyces diastaticus의 에탄올내성 증진

  • Song, Sang-Ho (Center For Genetic Engineering Research, The University of Suwon) ;
  • Kim, Keun (Center For Genetic Engineering Research, The University of Suwon) ;
  • Lee, Min-Woong (Department of Agrobiology, Dongguk university)
  • 송상호 (수원대학교 유전공학연구소) ;
  • 김근 (수원대학교 유전공학연구소) ;
  • 이민웅 (동국대학교 농생물학과)
  • Published : 1994.12.01

Abstract

Several mutation procedures have been compared to obtain an ethanol-tolerant Saccha- romyces diastaticus strain secreting glucoamylase. These procedures include spontaneous mutation, EMS treatment, UV irradiation, and combination of EMS treatment and UV irradiation. All these methods were followed by adaptation of the yeast cells to gradually higher ethanol concentration. Among these procedures, the combined method of EMS treatment and UV irradiation gave the promising result, i.e. the ethanol tolerance of the yeast increased from 11.5%(v/v) to 14.0%(v/v). Respiratory deficient petite mutants of industrial and ethanol-tolerant yeast strains have been isola- ted and hybridized with haploid S. diastaticus strains. The resulting hybrids showed increased ethanol tolerance and starch-fermentability.

Keywords

Starch-fermenting yeast;ethanol tolerance;mutation;rare-mating

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