Analysis of Lactobacillus casei and Mutant Strains by Polymerase Chain Reaction

Polymerase Chain Reaction에 의한 Lactobacillus casei 및 돌연변이 균주들의 비교 분석

  • Nam, Jin-Sik (Department of Biology, Keimyung University) ;
  • Lee, Jeong-Jun (Hankuk Yakult Institute) ;
  • Shin, Myeong-Su (Hankuk Yakult Institute) ;
  • Na, Seog-Hwan (Hankuk Yakult Institute) ;
  • Baek, Young-Jin (Hankuk Yakult Institute) ;
  • Yoo, Min (Department of Biology, Keimyung University)
  • 남진식 (계명대학교 생물학과) ;
  • 이정준 (한국야쿠르트유업(주) 연구소) ;
  • 신명수 (한국야쿠르트유업(주) 연구소) ;
  • 나석환 (한국야쿠르트유업(주) 연구소) ;
  • 백영진 (한국야쿠르트유업(주) 연구소) ;
  • 유민 (계명대학교 생물학과)
  • Published : 1994.12.01


To classify Lactobacillus casei strains on the basis of difference in their chromosomal DNA sequence, we have performed polymerase chain reactions on their chromosomal DNA by using random primers, and followed by analyzing randomly amplified polymorphic DNA fragments. We also developed a mini-preparative method to isolate PCR-grade chromosomal DNA from Lacto- bacillus casei strains within 3 hours. Based on RAPD pattems by polymerase chain reactions with degenerated random primers, 4 Lactobacillus casei strains and 2 mutant strains were successfully discriminated. Results were very sensitive, strain-specific and reproducible. It was also reliable. These results suggest that RAPD may be applied efficiently for the identification of several Lactoba- cillus casei strains.


Lactobacillus casei;random primer;polymerase chain reaction;RAPD


  1. Milchwissenschaft v.35 Effect of ingesting Lactobacillus acidophilus milk upon fecal flora and enzyme activity in human Ayebo,A.D.;I.A.Angelo;K.M.Shahani
  2. Med. Microbiol. Immunol. v.179 Augmentation of antimetastatic effect on Lewis lung carcinoma(3LL0) in C57BL/6 mice by priming with Lactobacillus casei Matsuzaki,T.;Y.Shimizu;T.Yokokura
  3. The polymerase chain reaction : an improved method for the analysis of nucleic acids v.83 Vosberg,H.
  4. Procl. Natl. Acad. Sci. USA. v.88 Rapid indentification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near isogenic lines Martin,G.B.;J.G.L.Williams;S.D.Tanksley
  5. J. Bacteriology v.174 Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers Kersulyte,D.;J.P.Woods;E.J.Keath;W.E.Goldman;D.E.Berg
  6. Appl. Envrion. Microbiol. v.58 Sensitive and species-specific deterction of Erwinia amylovora by polymerase chain reaction analysis Bereswill,S.;A.Pahl;P.Bellemann;W.Zeller;K.Geider
  7. Biology of Industrial Microorganisms Biology of the lactic, acetic, and propionic acid bacteria Moat,A.G.;Demain,A.L.(ed.);N.A.Solomon(ed.)
  8. Kor. J. Appl. Microbiol. Bioeng. v.13 Rapid and simple method for isolating plasmid DNA from lactic acid bacteria Bae,H.S.;Y.J.Baek;Y.K.Kim;M.Yoo;M.Y.Pack
  9. Korean J. of Anim. Sci. v.22 산성환경에서 Lactobacillus casei YIT 9018의 생존에 관한 연구 김현욱;유광현;이돈성;고준수
  10. Genetics Brown,T.A.
  11. Biotherapy v.1 Antitumor effect of Lactobacillus casei YIT 9018(LC9018) on mouse B16 melanoma and its highly metastatic variants Matsuzaki,T.;K.Tsukio;T.Yokokura
  12. Kor. J. Appl Microbiol. Bioeng v.14 A study on the protoplast formation and regeneration of Lactobacillus casei YIT 9018 Baek,Y.J.;H.S.Bae;M.Yoo;Y.K.Kim;H.U.Kim