Analysis of Lactobacillus casei and Mutant Strains by Polymerase Chain Reaction

Polymerase Chain Reaction에 의한 Lactobacillus casei 및 돌연변이 균주들의 비교 분석

  • Nam, Jin-Sik (Department of Biology, Keimyung University) ;
  • Lee, Jeong-Jun (Hankuk Yakult Institute) ;
  • Shin, Myeong-Su (Hankuk Yakult Institute) ;
  • Na, Seog-Hwan (Hankuk Yakult Institute) ;
  • Baek, Young-Jin (Hankuk Yakult Institute) ;
  • Yoo, Min (Department of Biology, Keimyung University)
  • 남진식 (계명대학교 생물학과) ;
  • 이정준 (한국야쿠르트유업(주) 연구소) ;
  • 신명수 (한국야쿠르트유업(주) 연구소) ;
  • 나석환 (한국야쿠르트유업(주) 연구소) ;
  • 백영진 (한국야쿠르트유업(주) 연구소) ;
  • 유민 (계명대학교 생물학과)
  • Published : 1994.12.01

Abstract

To classify Lactobacillus casei strains on the basis of difference in their chromosomal DNA sequence, we have performed polymerase chain reactions on their chromosomal DNA by using random primers, and followed by analyzing randomly amplified polymorphic DNA fragments. We also developed a mini-preparative method to isolate PCR-grade chromosomal DNA from Lacto- bacillus casei strains within 3 hours. Based on RAPD pattems by polymerase chain reactions with degenerated random primers, 4 Lactobacillus casei strains and 2 mutant strains were successfully discriminated. Results were very sensitive, strain-specific and reproducible. It was also reliable. These results suggest that RAPD may be applied efficiently for the identification of several Lactoba- cillus casei strains.

Keywords

Lactobacillus casei;random primer;polymerase chain reaction;RAPD

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