Isolation and characterization of Cellulose Producing Acetobacer xylinum KI Strain

Cellulose 생성 Acetobacter xylinum KI 균주의 분리 및 특성

  • Cha, Young-Ju ;
  • Park, Kyung-Jin ;
  • Kim, Do-Kyung ;
  • Chun, Hong-Sung ;
  • Lee, Byung-Kwon ;
  • Kim, Keun-Hyung ;
  • Lee, Sook-Young ;
  • Kim, Sung-Jun
  • 차영주 (조선대학교 유전공학과) ;
  • 박경진 (조선대학교 유전공학과) ;
  • 김도경 (조선대학교 유전공학과) ;
  • 전홍성 (조선대학교 유전공학과) ;
  • 이병권 (조선대학교 유전공학과) ;
  • 김근형 (동아전문대학 공업화학과) ;
  • 이숙영 (조선대학교 유전공학과) ;
  • 김성준 (조선대학교 유전공학과)
  • Published : 1994.12.01

Abstract

One strain of cellulose-producing Acetobacter was isolated from the traditionally fermen- ted grape vinegar in Korea. The isolated strain, designated as KI strain was identified as the Acetobacter xylinum with respect to physiological and biochemical characteristics. KI produced acetic acid from ethanol, and then decomposed acetate to CO$_{2}$ and H$_{2}$O. When the isolated strain was cultivated statically in broth culture, a thick cellulose pellicle was formed. KI was tolerance of 8% ethanol and 30% glucose, and the isolate was positive in ketogenesis from glycerol, $\gamma$-pyrone from glucose and fructose, and 2-ketogluconic acid from glucose. KI strain possessed straight-chain C$_{18:1}$, C$_{16:0}$, and C$_{14:0}$ fatty acid, and contained ubiquinone Q$_{9}$ and Q$_{10}$ as isoprenoid quinone. DNA base composition of KI strain was 57.6% G+C.

Keywords

Acetobacter xylinum;cellulose-producing bacteria;characterization

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