Pullulan Production and Morphological Change of Aureobasidium pullulans ATCC 9348

Aureobasidium pullulans ATCC 9348의 Pullulan 생산과 균체 형태의 변화

  • Kwon, Oh-Sung ;
  • Nam, Hee-Sop ;
  • Lee, Hyung-Jae ;
  • Shin, Yong-Chul
  • 권오성 ((주) 농심 기술개발연구소) ;
  • 남희섭 ((주) 농심 기술개발연구소) ;
  • 이형재 ((주) 농심 기술개발연구소) ;
  • 신용철 (경상대학교 미생물학과)
  • Published : 1994.12.01

Abstract

The pullulan production and morphological change of Aureobasidium pullulans ATCC 9348 were investigated both in batch fermentation and in continuous fermentation. The best carbon source for pullulan production was sucrose among seven different carbon sources. The pullulan production of A. pullulans was increased with increasing the carbon to nitrogen ratio of the medium using sucrose as a carbon source. In batch fermentation, production of pullullan occurred following exhaustion of the nitrogen source from the medium. The continuous fermentation showed that the pullulan production was closely parallelled with cell growth and was most effective at a dilution rate of 0.06~0.07 hr$^{-1}$-. The ratio of yeast-like cells(blastospores) of A. pullulans increased with the increase of growth rate, and reached 100% over the growth rate of 0.07 hr$^{-1}$. The growth rate, within a certain range, affected not only on the cell morphology, but on the specific pullulan productivity of A. pullulans.

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