Media Optimization for L-tryptophan Production by Genetically Engineered Klebsiella pneumoniae

유전자 조작한 Klebsiella pneumoniae로부터 L-트립토판 생산을 위한 발효배지 조건

  • 김천규 (고려대학교 화학공학과) ;
  • 정용섭 (전북대학교 식품공학과) ;
  • 홍석인 (고려대학교 화학공학과)
  • Published : 1994.10.01

Abstract

The optimum medium composition for the production of L-tryptophan with Klebsiella pnuemoniae pheA tyrA trpE trpR/pSC 101-trp$^{+}$ and the effect of precusors in the optimum medium were studied. The specific growth rate in the optimum medium was almost the same as that in the basal medium, the former showing 1.01 and the latter 1.07 hr $$^{-1}$, but the produced tryptophan was increased 45% in the optimum medium. The maximum amount of produced tryptophan was 159 mg/l within 14 hours. Tryptophan production was ceased by casamino acid addition over 4 g/l in medium, but cell maSS increased with its addition. Indole and anthranilate as precusors had toxic effect on growth and tryptophan production at experimented concentration range (over 20 mg/l), but L-serine had good effect on tryptophan production, resulting in 175 mg/l tryptophan within 14 hours.

Keywords

Klebsiela pneumoniae;L-tryptophan;culture conditions

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