Production and Characterization of ans Alkaline Protease from an Isolate,Xanthomonas sp.YL-37

알칼리성 Prottease를 생산하는 Xanthomonas sp. YL-37의 분리 및 조효소의 성질

  • Lee, Chang-Ho ;
  • Kwon, Tae-Jong ;
  • Kang, Sang-Mo ;
  • Suh, Hyun-Hyo ;
  • Kwon, Gi-Seok ;
  • Oh, Hee-Mock ;
  • Yoon, Byung-Dae
  • 이창호 (한국과학기술연구원 유전공학연구소) ;
  • 권태종 (건국대학교 미생물공학과) ;
  • 강상모 (건국대학교 미생물공학과) ;
  • 서현효 (한국과학기술연구원 유전공학연구소) ;
  • 권기석 (한국과학기술연구원 유전공학연구소) ;
  • 오희목 (한국과학기술연구원 유전공학연구소) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소)
  • Published : 1994.10.01

Abstract

A bacterial strain, which showed the high protease activity at low temperature and the high tolerance for the surfactant, was isolated from soil and identified as Xanthomonas sp. YL-37. The optimal temperature, initial pH, and cultivation time for the production of the alkaline protease by Xanthomonas sp. YL-37 were 20$\circC , 11.0, and 84 hours, respectively. In the jar fermenter culture of Xanthomonas sp. YL-37, the alkaline protease activity was about 15,000 DU/ml/-broth after cultivating for 108 hours. The optimal pH and temperature for the protease activity were 70$\circC and 11.0, respectively. The protease was relatively stable at the pH range of 7.0~12.0 and at the temperatures below 50$\circC . The protease activity at 20$\circC was about the level of 40% of its activity at 70$\circC . The enzyme was suggested as a serine protease because the enzyme activity was inhibited by phenylmethane sulfonyl fluoride, a serine modifier.

Keywords

Xanthomonas sp. YL-37;alkaline protease

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