Saccharification Using Pullulanase from Klebsiella pneumoniae

Klebsiella pneumoniae NFB-320이 생산하는 Pullulanase를 이용한 전분당화

  • Kwon, Jay-Min ;
  • Park, Kyung-Ho ;
  • Pek, Un-Hwa ;
  • Bai, Dong-Hoon ;
  • Yu, Ju-Hyun
  • 권재민 (연세대학교 식품생물공학과) ;
  • 박경호 (두산기술원) ;
  • 백운화 (두산기술원) ;
  • 배동훈 (단국대학교 식품공학과) ;
  • 유주현 (연세대학교 식품생물공학과)
  • Published : 1994.10.01

Abstract

In order to convert starch to the fermentative sugar, the effect of pullulanase on the saccharification of starch and pullulanase was investigated. The optimum pH and temperature for the enzyme activity of the glucoamylase and the crude pullulanase from Klebsiella pneumoniae NFB-320 were shown to be identical as pH 6.0 and 60$\circC, respectively. The crude pullulanase was stable between pH 5.0~6.5, and up to 40$\circC, whereas the glucoamylase was stable between pH 4.0~6.5, and up to 40$\circC. When pullulanase and glucoamylase were engaged together in the sacchrification of starch, saccharification yield was increased by 3.2% than the yield obtained by glucoamylase, alone. And the two enzymes produced sugar from pulltulan 18 times much higher than the single use of pullulanase.

Keywords

Saccharification;pullulanase;Klebsiella pneumoniae

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