A Gap Phase-Specific Inhibitor of the Mammalian Cell Cycle from Streptomyces sp. ZF10

Streptomyces sp. ZF-10이 생산하는 세포주기 저해제

  • ;
  • ;
  • Hiroyuki Osada
  • 하상철 (한국과학기술연구원 유전공학연구소) ;
  • 홍순덕 (경북대학교 자연과학대학 미생물학과) ;
  • Published : 1994.10.01

Abstract

Genistein, a inhibitor of the progression of G$_{1}$ and G$_{2}$ phase of the mammalian cell cycle, was discovered through a unique screening system, in which effects of microbial metabolites on the cycle progression of the cultured mouse mammalian carcinoma cell were monitored by flow cytometry. The inhibitor was extracted from the fermentation broth of Streptomyces sp. ZF10 with ethyl acetate, and purified by silica gel column chromatography and HPLC.

Keywords

Cell cycle inhibitor;genistein;Streptomyces sp.

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