Gene Expression and Secretion of Human Epidermal Growth Factor in a Methylotrophic Yeast Hansenula polymorpha

메나놀 자화 효모 Hansenula polymorpha를 이용한 재조합 인체 표피 성장인자 유전자의 발현 및 분비

  • Oh, Yong-Ik ;
  • Sohn, Jung-Hoon ;
  • Choi, Eui-Sung ;
  • Kim, Hee-Chul ;
  • Rhee, Sang-Ki
  • 오용익 ((주) 녹십자 종합연구소) ;
  • 손정훈 (한국과학기술연구원 부설 유전공학연구소, 응용미생물연구그룹) ;
  • 최의성 (한국과학기술연구원 부설 유전공학연구소, 응용미생물연구그룹) ;
  • 김희철 ((주) 녹십자 종합연구소) ;
  • 이상기 (한국과학기술연구원 부설 유전공학연구소, 응용미생물연구그룹)
  • Published : 1994.10.01

Abstract

Using a methylotrophic yeast Hansenula polymorpha, a heterologous gene expression and secretion system was developed for the production of hEGF(human Epidermal Growth Factor) which has been shown to promote epithelial cell proliferation and to inhibit gastric acid secretion. The hEGF gene was chemically synthesized according to the preferred codon usage in H. polymor- pha and expressed under the control of the strong and inducible methanol oxidase(MOX) promoter. The mating factor $\alpha$ pre-pro leader sequence of Saccharomyces cerevisiae was employed for hEGF to be secreted into the extracellular medium. This expression cassette was stably integrated into the host chromosomal DNA. Mature hEGF was efficiently expressed and secreted into the extracel- lular medium. About 24 mg/l of hEGF was detected in the cuture supernatant of a transformant with pA-EGF3 under the suboptimal culture conditions.

Keywords

Human Epidermal Growth Factor(hEGF);Hansenula polymorpha;Expression and secretion

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