Characterization of Prophange Cured Strain Derivative from Lactobacillus casei YIT 9018

Lcatobacillus casei YIT 9018로 부터 분리한 Prophage Cured Strain의 특성

  • Lee, Jeong-Jun ;
  • Oh, Tae-Kwang ;
  • Chang, Hyo-Ihl ;
  • Baek, Young-Jin
  • 이정준 (한국 아쿠르트 유업(주) 연구소) ;
  • 오태광 (KIST, 유전공학연구소 응용미생물 연구그룹) ;
  • 장효일 (고려대학교 자연자원대학 유전공학과) ;
  • 백영진 (한국아쿠르트 유업(주) 연구소)
  • Published : 1994.10.01

Abstract

Lactobacillus casei HY 2782, prophage cured strain was characterized to be stable as much as L casei YIT 9018, parent strain. By southern hybridization, it was confirmed that the temperate phage was incorporated in chromosomal DNA of L. casei YIT 9018 as a prophage. It was also proved that the prophage was cured from chromosomal DNA of L casei HY 2782. The growth rate, lactic acid producing ability, carbohydrates fermentation, and enzymatic activity of L. casei HY 2782 were found to be similar to those of L. casei YIT 9018. When L casei HY 2782 was used as a host, the multiplicity of infection (M.O.I.) of the temperate phage for L. casei HY 2782 was 1.0~5.0. Restriction enzyme analysis of pLC90 plasmid from L. casei HY 2782 was shown that the size was an approximately 68.22 kb. The plasmid profiles, genomic DNA patterns, and cellular fatty acids composition of L. casei HY 2782 were similar to those of L casei YIT 9018. And the major fatty acids composition of these strains were C$_{14;0}$,C$_{16;1}$, C$_{16;0}$, C$_{18;1}$ and C$_{19;cyclo-}$ 10 sets of arbitrary primer in the PCR were screened to find differentiation against two strains of L. casei. Among them, b$_{5}-1/17-1 primer was produced an approximately 1.3 kb DNA band of only L casei YIT 9018. And b$_{5}-2/17-2 primer was produced an approximately 1.0 kb DNA band of only L casei HY 2782.

Keywords

Lactobacillus casei YIT 9018;prophage cured strain;Lactobacillus casei HY 2782;characterization

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